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Covaris
E220 & LE220 (-plus, R-plus, Rsc) Setup & Instruction Manual
Part Number: 010521 Rev B | May 2021
Using Position Restrictions When Running Methods
It is sometimes convenient to run a method on a plate that is only partially populated with samples. For example, consider a method
that treats all 12 columns in a 96-well plate. But now you have a plate that has samples only in columns 4 to 6. Using Position
Restrictions allows treating a subset of a method’s specified positions. See instructions in
Appendix B
of this document, Using Position
Restrictions.
To modify an existing method, refer to
Section 5
: Create and Edit Methods.
3.4 System Shutdown
If the instrument will be idle for an extended period of time (e.g. longer than two weeks), refer to the system shutdown procedure in
the User Maintenance section of this manual.
When your system is finished being used for the day, leave the transducer submerged in the bath if automatic scheduling is active.
At the end of the scheduled period, the scheduler will turn off the degas pump and disable cooling in the chiller, then automatically
restart both as scheduled for the next day. SonoLab software must be left running and the system and CH05 chiller powered on for
the scheduled events to occur.
If scheduling is not in use, click on the ‘Water Control’ button and then the ‘Click for Standby’ button. The transducer will rise out
of the bath and the CH05 chiller’s heater/cooler will turn off, but the chiller’s pump will continue to operate, circulating bath water
through the Water Conditioning System (WCS).
NOTE
: No scheduled events will occur when the instrument is in “Standby” mode, regardless of whether or not SonoLab is
in scheduled mode.
CAUTION
: Do not turn off the WCS or CH05 chiller. These must remain powered on to treat the bath water against bio-
growth. Leaving water in the water bath for an extended time without the WCS operating may promote algal growth in the
bath and lines. This growth can interfere with the water quality and sample processing.
