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Covaris
E220 & LE220 (-plus, R-plus, Rsc) Setup & Instruction Manual
Part Number: 010521 Rev B | May 2021
• All treatment steps will be applied to all selected columns (samples) of the plate once and then repeated for the number of times
specified in the Repeat bar. That is, both programmed Treatments will be given to column 1 (sample A1), then both Treatments
will be given to column 2 (sample B1), and so forth until the entire plate is treated and then the treatment steps are repeated.
• When all method parameters and steps have been defined, click on Save to save changes.
6.3 Scanning Treatments (LE220Rsc Only)
In a scanning treatment, rather than the sample tray indexing to place one column of the sample tray at a time over the transducer, the
sample tray moves continuously over the transducer. In this mode, acoustic dose is a function of the speed at which the sample tray
moves and the number of passes. One or more rows may be treated, but the rows must be adjacent.
6.3.1 Create a scanning treatment:
• Click the “add step” button.
• Select Repeat from the step type list.
Enter the desired number of scans in the “Iterations” field. One iteration passes the sample tray over the transducer one time. If only a
single scan step is desired, the ‘Repeat’ is not necessary and a ‘Scan’ step may be selected here and the next two steps below skipped.
