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Covaris
E220 & LE220 (-plus, R-plus, Rsc) Setup & Instruction Manual
Part Number: 010521 Rev B | May 2021
Treatment parameters are chosen by directly editing the value boxes. The treatment parameters are:
•
Peak Power (PIP):
This parameter measures the peak electrical power used to drive the ultrasound transducer. The adjustable
range of Peak Incident Power is 1.5 to 500.0 Watts.
•
Duty Factor (DF):
This parameter defines the ratio between the on-time of the transducer and total operation time and is
expressed as a percentage. For a given Peak Incident Power, a larger Duty Factor results in more acoustic energy generated
from the transducer. Users can specify a Duty Factor from 1 to 50%.
The product of the Peak Power and Duty Factor gives the approximate Average Incident Power (AIP) generated from the
transducer. For example, if Peak Power is set to 10 Watts and Duty Factor is set to 15%, the Average Incident Power will be
approximately 1.5 Watts. However, the real Average Incident Power from the transducer may vary due to factors such as
sample tube type, water level and water temperature.
The maximum Average Incident Power (AIP), the product of PIP times Duty Factor (DF) is limited to 250 watts on all E220-
plus and LE220-plus instruments.
•
Cycles per Burst (CPB):
This parameter indicates the number of electrical signals used in each acoustic “burst” of the
transducer. The range of Cycles per Burst (CPB) is an integer from 50 to 1000 for different applications. Advanced users
may use this parameter to fine tune their experiments.
•
Duration:
This parameter defines the amount of time for which the sample is under a treatment. The measurement of the
Duration is in seconds. The maximum time for any treatment is 3600 seconds. If a longer treatment is necessary, “Repeat
Steps” can be used to extend the treatment.
SonoLab displays the Average Incident Power, calculated from the Peak Incident Power and Duty Factor, adjacent to the treatment
parameters in the treatment step bar. The maximum Average Incident Power is 250 Watts.
If a parameter exceeding the limits is entered, a red outline is displayed around the entry and a message is displayed in the “Steps” bar,
as shown below.
In this example, 600 watts exceeds the 500 limit for PIP. The method Save button is disabled until the parameters are corrected.
