MVE Biological Systems
TEC 3000 Operating and Maintenance Freezer Manual
13289499 Rev G •
6
Equipment Usage
Cryogenic containers must be operated in accordance with the manufacturer/supplier instructions.
Cryogenic Dewars must be kept in a well- ventilated area protected from weather and away from heat
sources. In applications that use a modular liquid cylinder as a source of LN2, the supply will need to be
replenished at regular intervals to ensure proper operation of the freezer. When exchanging liquid
cylinders, follow the below procedure:
1. Allow all plumbing components to warm to room temperature before attempting to
change supplies.
2. Close all valves associated with the liquid supply cylinder.
3.
Relieve pressure in the plumbing assembly by initiating a brief fill by either pressing “Start
Fill” or using the manual fill button.
4. Loosen the plumbing connection for the transfer hose at the liquid cylinder.
5. Remove empty liquid cylinder and replace with full liquid cylinder pressurized to 22 - 35
psig (1.52 - 2.41 bar).
6. Attach the transfer hose to the plumbing connection on the liquid cylinder. Ensure that the
hose is connected to the connection labeled
“LIQUID”.
7. Tighten the transfer hose plumbing connection at the liquid cylinder.
8. Open the liquid supply valve on the liquid cylinder.
9. Inspect plumbing for audible and visual leaks. Repair if necessary.
10. Manually initiate a fill to verify proper operation.
Recommended First Aid
Every site that stores and uses LN2 should have an appropriate Material Safety Data Sheet (MSDS)
present. The MSDS may be obtained from the LN2 distributor. The MSDS will specify the symptoms of
overexposure and first aid to be used. Here is a typical summary.
If symptoms of asphyxia such as headache, drowsiness, dizziness, excitation, excess
salivation, vomiting, or unconsciousness are observed, remove to fresh air. If breathing has
stopped, give artificial respiration.
CALL A PHSYICIAN IMMEDIATELY.
If breathing is
difficult, supplemental oxygen maybe required. If exposure to cryogenic liquids or cold vapor
occurs, restore tissue to normal, body temperature (37°C) as rapidly as possible, and then
protect the injured tissue from further damage and infection.
Rapid warming of the affected areas is best achieved by bathing it in warm water. The
temperature of the water used should not exceed 40°C. Under no circumstances should the
frozen part be rubbed either before or after warming. If the eyes are involved, flush them
thoroughly with warm water for at least 15 minutes. In case of massive exposure, remove
clothing while showering with warm water. The patient should not drink alcohol or smoke.
CALL A PHYSICIAN IMMEDIATELY
.
