MVE Biological Systems
TEC 3000 Operating and Maintenance Freezer Manual
13289499 Rev H •
29
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Remove a caplug from the fill tee on the plumbing assembly in order to connect the LN2 supply. If
using an LN2 cylinder as the liquid supply, securely connect and tighten the transfer hose to both
the fill tee connection and the supply connection labeled “LIQUID”. If using a bulk LN2 supply
system, securely connect and tighten the supply connection to the freezer fill tee connection.
NOTE: The recommended LN2 supply pressure is 22-35 psig (1.52 - 2.41 bar).
First Fill Startup procedure
1. Once the freezer has been properly installed, begin the first fill procedure. Fill freezer at the
desired location where it is to be used. Do not fill to move freezer to another location.
2. Open or remove the lid for the first fill due to the accelerated LN2 evaporation rate when filling a
warm freezer. If the freezer is equipped with a lid switch, engage the manual override (see the Lid
Switch section of this manual for more info).
3. Verify supply pressure at 22-35 psig (1.52
– 2.41 bar) open valve and press “Start Fill” to begin
filling the freezer.
4. The first fill will take significantly longer than subsequent fills due to a warm freezer.
5. It is recommended to place empty inventory system components such as racks, boxes, frames, or
canisters in the freezer during the first fill. This will cool the inventory system as well as help the
freezer reach its top box temperature rating faster.
6. It is normal for some condensation or frost to develop around the neck opening during the initial
fill.
7. Once the TEC3000 reads a level; press stop fill; allow liquid to equalize and insert dipstick; verify
level to the controller level display; change the offset value (+ or -) to match liquid level; press
start fill to continue the fill process. When freezer has reached its high level set point wait 10
minutes for system to equalize then verify level with dipstick and calibrate if necessary.
8. Once the TEC3000 matches freezer LN2 level close or place the lid and allow the freezer to
equilibrate and reach its temperature rating.
9. It is recommended that biological samples are not introduced into the freezer until several days
after the top box temperature has stabilized at or below the freezer’s temperature rating. It is
recommended to restart controller after 24 hours of initial install to reset the Liquid Usage.
10. Refer to the Operations section of this manual for further instructions.
Note: Pressing Stop Fill will disable TEC3000’s automatic fill function for 30 minutes.
