Biotek ELx808, Operator'S Manual, Page: 71 / 172

Defining Assays
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49
Reuse of Standard Curves
The ELx808 has the ability to reuse a standard curve that has already been
established.
Limitations of the Reuse of Standard Curves
• The reuse of standard curves can only be done in assay positions 31
through 55. Each of these positions can only store one standard curve.
•
Standard curves cannot be reused on panels (see page 67 for Panel
Assays
).
•
Standard curves will be stored in memory with the Assay Name, Standard
Concentrations, Replicate Counts, and Optical Densities for each standard
replicate.
• Stored standard curves can only be reused for the assay that the curve was
originally generated on (i.e., the curve for assay #53 cannot be applied to
samples on a plate to be run in assay #51).
•
To reuse a standard curve, an assay must first be programmed (in positions
31 through 55) and run. During the defining process, you will be prompted
to enter the number of standards, the number of standard replicates, and
the standard concentrations
. The following screen has been added after
these prompts:
R E U S E S T A N D A R D C U R V E ? Y E S
Y E S N O
• After the assay has been run, the results have been calculated, and the
reports have been generated, the reader will prompt if this standard curve
should be stored in memory. The following display will appear:
S A V E S T A N D A R D C U R V E ? Y E S
Y E S N O
•
Select YES to store the curve for use at a later time. The next time a plate is
to be read using this assay, the instrument will prompt if there are
standards on the plate. Select
NO to discard the curve.
ELx808 Operator’s Manual