Biotek ELx808, Operator'S Manual

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Sample Anticardiolipin IgG Enzyme Immunoassay Kit | 139
Criteria Range (GPLU/mL)
High Positive > 80
Medium Positive ≥ 20-80
Low Positive ≥ 10, < 20
Results Interpretation
The association between low positive levels of anticardiolipin antibodies and clinical
findings is unclear.
Normal population studies indicate that there is a higher prevalence of IgM positives
in the normal population than IgG, 9.4% and 6.5%, respectively. In normal pregnancy,
the levels are higher still at 17.0% (IgM) and 10.6% (IgG).
Expected Values
The normal range was determined on serum from 102 normal adult blood donors. The
ranges below are provided as a guide only. ELISA assays are very sensitive and
capable of detecting small differences in sample populations. It is recommended that
each laboratory determine its own normal range, based on the population techniques
and equipment employed.
lgG Anticardiolipin
< 11 GPL U/mL Negative result
> 11 GPL U/mL Positive result
Programming the Assay on the ELx808
From the Main Menu, press DEFINE . Enter the assay number and edit the name if
desired. At the DEFINE menu, follow the steps below:
STEP
To program the reading method, press:
METHOD:
READ TYPE: Endpoint
DELAY FIRST READ:
If incubator model: INCUBATION TEMP (Ambient or Temperature):
DELAY FIRST READ:
ELx808 Operator’s Manual