18
Biotage
®
Flash 75/150 User Manual | © Biotage 2018
How to Use the System
15. Ensure that the fraction collection nozzle is inserted into a
collection vessel of a suitable size. See the guidelines above.
Note:
If using a solid sample, the sample has not yet been
loaded onto the cartridge. It will be loaded when you open
the two way valve on the solvent reservoir in the next step.
16. Open the two-way valve on the solvent reservoir. Solvent
will then flow through the SIM and cartridge (solid sample)
or cartridge (liquid sample) and out of the fraction collection
nozzle.
17. Once your separation is finished, stop the solvent flow by
closing the two-way valve on the solvent reservoir.
18. Turn the
MV-03
valve on the AM-190 manifold to its off/vent
position (see Table 2 on page 3).
19. Close the three-way valve on the radial compression module.
Refill the Solvent Reservoir
If you need to refill the solvent reservoir during a run:
1. Close the two-way valve on the solvent reservoir.
2. Depressurize the solvent reservoir by turning the
MV-03
valve on the AM-190 manifold to its off/vent position (see
Table 2 on page 3).
3. When the pressure has been released, open the lid by
loosening the clamp screw and then tilting the lid.
4. Ensure that system is grounded; see “Ground the System”
on page 12.
5. Take the necessary precautions to avoid exposure to
harmful gases and then fill the solvent reservoir with the
desired solvent.
Note:
If changing solvent, the new solvent must be miscible
with the previous solvent.
6. Put the lid back in place by tilting and lowering it into the
mouth of the solvent reservoir. Hand-tighten the clamp to
secure the lid.
Empty the Solvent Reservoir
Warning
»
Only use waste/fraction collection vessels that are grounded or
made of glass.
If you want to empty the solvent reservoir after the run, this is
preferably done before depressurizing the radial compression
module.
1. Ensure that the three-way valve on the radial compression
module and the two-way valve on the solvent reservoir are
closed.
2. Disconnect the solvent tube from the radial compression
module and insert it into a collection vessel of a suitable size.
3. Turn the
MV-03
valve
on the AM-190 manifold to its
on/pressurize position (see Table 2 on page 3).
4. Lower the solvent pressure to 15 psi (1 bar).
5. Take the necessary precautions to avoid exposure to
harmful gases.
6. While firmly holding down the solvent tube into the
collection vessel, slowly open the two-way valve on the
solvent reservoir.
Depressurize the Radial Compression
Module and Remove the Used Cartridge
Warning
»
Never loosen the V-band clamps when the radial compression
module is under pressure. A red pressure indicator on the radial
compression module sticks out when the pressure is above
20 psi (1.4 bar); see Figure 2 on page 2.
»
Only use waste/fraction collection vessels that are grounded or
made of glass.
1. If using a Flash 150 system, reduce the solvent pressure to
less than 50 psi (3.5 bar) by adjusting the
PRR-02
regulator
before blowing down the radial compression module.
2. Insert the fraction collection nozzle into a collection vessel
that are grounded or made of glass.
3. Turn the three-way valve on the radial compression module
toward the tube connected to the SIM.
4. Turn the three-way valve on the SIM toward the BLUE tubing
that is connected to the AM-190 manifold.
5. Turn the
MV-02
valve on the AM-190 manifold to its
on/pressurize position (see Table 2 on page 3)to blow
inert gas through the SIM and cartridge and drive out any
remaining solvent.
6. When the last of the solvent drains out of the radial
compression module, turn the
MV-02
and
MV-01
valves on
the AM-190 manifold to their off/vent positions (see Table 2
on page 3).
7. When the radial compression module is not under pressure,
i.e. the red pressure indicator on the front of module is not
sticking out (see Figure 2 on page 2), turn off the inert
gas supply to the system and allow any pressure to dissipate
through the system.
8. Loosen the upper V-band clamp and remove the top head
assembly from the radial compression module.
9. Remove the cartridge using the cartridge extraction tool
supplied with the system; see Figure 25.
10. Put the end caps back on the cartridge and dispose of it in a
safe manner.
Summary of Contents for Flash 150
Page 1: ...Biotage Flash 75 150 User Manual ...
Page 34: ...32 Biotage Flash 75 150 User Manual Biotage 2018 Notes Notes ...
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