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15
How to Use the System
Figure 18. Inserting a flash cartridge into the radial compression module.
Pressurize the Radial Compression
Module and the Solvent Reservoir
Warning
»
The system is operated using pressurized inert gas. Ensure that
all seals are properly mounted and that all clamps are properly
closed before introducing pressurized gas into the system.
»
To avoid injury to yourself or damage to the system, set
the inert gas supply pressure to between 100 and 125 psi
(6.9 to 8.6 bar).
»
Follow all applicable safety procedures when working with
bottled gas.
1. Ensure the system is grounded; see “Ground the System” on
page 12.
2. Ensure that the three-way valve on the radial compression
module and the two-way valve on the solvent reservoir are
closed.
3. Ensure that all the lines are properly connected as described
in “Attach the Inert Gas Supply and Solvent Lines” on
page 10 and that there is solvent in the solvent reservoir.
Note:
If using a SIM, the solvent tube must be connected
between the solvent reservoir and the radial compression
module as shown in the left schematic in Figure 13 on
page 11 for you to be able to equilibrate the cartridge.
The solvent tube may contain solvent; please ensure to
take the necessary precautions to avoid spillage when
disconnecting the tube.
4. Turn on the inert gas supply that is connected to the AM-190
manifold (see A in Figure 4 on page 3). Confirm that the
gas pressure is between 100 and 125 psi (6.9 to 8.6 bar).
5. Pressurize the radial compression module by turning the
MV-01
valve on the AM-190 manifold to the on/pressurize
position (see Table 2 on page 3).
6. Set the radial compression pressure to 100 psi (6.9 bar) by
adjusting the
PRR-01
regulator on the AM-190 manifold.
7. Confirm that the cartridge is compressed; the red pressure
indicator on the front of the radial compression module
sticks out (see Figure 2 on page 2).
8. Pressurize the solvent reservoir by turning the
MV-03
valve
on the AM-190 manifold to the on/pressurize position (see
Table 2 on page 3).
9. Set the equilibration flow rate/solvent pressure by adjusting
the
PRR-02
regulator on the AM-190 manifold as follows:
»
Flash 75M: approx. 30 psi (2.1 bar)
»
Flash 75L and Flash 150M: approx. 45 psi (3.1 bar)
»
Flash 150L: approx. 45 to 60 psi (3.1 to 4.1 bar)
Note:
These solvent pressure settings are approximate
guidelines. Your particular mobile phase, depending on its
viscosity, may require a higher or lower pressure.
Note:
Ensure that the solvent pressure is at least 20 psi
(1.4 bar) lower than the radial compression pressure.
Equilibrate the Cartridge
Warning
»
Always equilibrate the cartridge.
»
Equilibrate the cartridge at a low flow rate to avoid extreme
temperature rise.
»
Only use waste/fraction collection vessels that are grounded or
made of glass.
»
If the system is placed in a walk-in fume hood, a fume extractor
must be used for open waste/fraction collection vessels.
1. Ensure that the fraction collection nozzle is inserted into a
collection vessel that is grounded or made of glass. If the
system is placed in a walk-in fume hood, a fume extractor
must be used for open collection vessels.
2. Turn the three-way valve on the radial compression module
toward the tube connected to the solvent reservoir.
3. Open the two-way valve on the solvent reservoir and allow
solvent to flow through the cartridge.
4. When solvent exits from the fraction collection nozzle, close
the two-way valve on the solvent reservoir and empty the
collection vessel.
Summary of Contents for Flash 150
Page 1: ...Biotage Flash 75 150 User Manual ...
Page 34: ...32 Biotage Flash 75 150 User Manual Biotage 2018 Notes Notes ...
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