4
Biotage
®
Flash 75/150 User Manual | © Biotage 2018
System Components
Solvent Reservoir
Biotage provides an electropolished 316 stainless-steel solvent
reservoir, available in a 12-liter capacity for the Flash 75 system
and 37-liter and 60-liter capacities for the Flash 150 system.
The solvent reservoir is fitted with an instant-tight fitting for
pressurized inert gas, an on/off valve for solvent flow, and
a safety relief valve that activates at pressures in excess of
109 psi (7.5 bar).
The oval lid of the reservoir is fitted with a clamp that screws
shut, and has a solvent proof, PTFE-encapsulated Viton O-ring
to seal the lid in place. See “Set Up the Solvent Reservoir” on
page 10 for safety precautions on how to secure the lid once
the reservoir has been filled with solvent.
Figure 5
Fitting for
inert gas
On/off valve
Safety relief
valve
Lid with clamp
. The 60-liter solvent reservoir with the two-way valve open.
Sample Injection Module (Biotage
®
SIM) Kit
Biotage provides a sample injection module (SIM) kit, available
in 500 mL, 1000 mL, and 2000 mL sizes, to simplify the handling
of routine samples as well as viscous oils and samples with
poor solubility that need to be adsorbed directly onto silica.
SIM 500 is optimized for Flash 75M and 75L, SIM 1000 for
Flash 150M, and SIM 2000 for Flash 150L.
Illustrated in Figure 6, the SIM body is constructed of a welded
316 stainless steel tube with a sanitary clamp at the top and a
stainless steel 1/8" (Flash 75) or 1/4" (Flash 150) tube fitting at
the bottom. The top lid comes with a three-way valve. One port
supplies inert gas to drive liquid sample onto the cartridge.
The other port supplies solvent through the SIM when using a
pre-adsorbed sample loading technique.
The SIM is supplied with six sanitary gaskets (two of each type,
see “Sanitary Gaskets” below), one chain clamp (P/N 01520),
25 top frits (P/N FTF-0225), 25 bottom frits with sealing rings
(P/N FBS-1025), and one frit insertion tool (P/N 01596 for
SIM 500 and 1000 and P/N 03339 for SIM 2000).
Figure 6
Three-way valve
Solvent flow
Inert gas
Sanitary
clamp
Frit holder
316 stainless
steel tube
Pour in liquids, oils,
or coated samples
To the radial compression module
Nut
. The sample injection module (SIM) simplifies the handling of
routine samples as well as viscous oils and samples with poor solubility.
Sanitary Gaskets
The SIM includes three different types of sanitary gaskets;
PTFE/EPDM, Viton, and EPDM (see Figure 7). Biotage recommends
the black and gray PTFE/EPDM gasket for most applications, as
it is resistant to most normal phase solvents and mixtures. The
gasket is moderately soft and seats when the sanitary clamp is
firmly tightened. The PTFE/EPDM gasket may leak a small amount
of solvent initially if the SIM is completely full of liquid.
The black Viton and EPDM gaskets are very compressible and
will seal reliably up to 100 psi (6.9 bar), even with a finger-tight
sanitary clamp. EPDM, which has a green color dot on the edge,
is resistant to polar solvents but will swell in many common
aliphatic and aromatic solvents. Viton, which has yellow and
white color dots on the edge, is resistant to non-polar solvents,
but will swell in the typical polar modifiers. For solvent
compatibility information, see Table 3 below.
Solvent
PTFE/EPDM
Viton
EPDM
Hexane
FAIR
GOOD
POOR
Toluene
GOOD
GOOD
POOR
Ethyl acetate
GOOD
POOR
GOOD
Dichloromethane
FAIR
FAIR
FAIR
Methanol
GOOD
POOR
GOOD
Table 3. Solvent compatibility.
Table 3 shows a limited number of commonly used solvents.
To check the compatibility of a particular solvent or solvent
mixture, soak a gasket in the solvent/mixture for one hour and
then inspect it for swelling. If the gasket swells in use, allow
it to dry out in a fume hood until it returns to its normal size
Summary of Contents for Flash 150
Page 1: ...Biotage Flash 75 150 User Manual ...
Page 34: ...32 Biotage Flash 75 150 User Manual Biotage 2018 Notes Notes ...
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