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3
System Components
Head Assemblies
Two stainless steel head assemblies, with knife-edges for
sealing the cartridge, attach to the top and bottom of the barrel
by means of V-band clamps, which can be easily hand-tightened
to seal the flash cartridge inside the barrel. The top head
assembly is fitted with a three-way valve that connects to the
solvent reservoir and the outlet of the Biotage sample injection
module (Biotage
®
SIM). The bottom head assembly has a tube
compression fitting; this is the fraction collection outlet of the
radial compression module.
AM-190 Manifold
The AM-190 manifold (see Figure 4) regulates the radial
compression pressure and the flow of sample and solvent into
the radial compression module. The manifold is easily attached
to standard fume hood mounting bars using the two mounting
rods supplied with the manifold; see “Set Up the AM-190
Manifold” on page 9.
The AM-190 manifold comes equipped with the following
features. See the corresponding letters in Figure 4.
A. An inlet for pressurized inert gas, with an instant-tight fitting
for attaching tubing.
B. A safety relief valve that activates at pressures in excess of
150 psi (10.3 bar).
C. A locking pressure regulator (labeled
PRR-01
), with a water
trap (C2), for increasing or decreasing the inert gas pressure
to the radial compression module.
D. A pressure gauge (labeled
PG-01
) for monitoring the inert
gas pressure delivered to the radial compression module.
E. A valve (labeled
MV-01
) for turning the flow of compressed
inert gas to the radial compression module on or off (see
Table 2), with an instant-tight fitting for attaching tubing (E2).
F. A locking pressure regulator (labeled
PRR-02
) for increasing
or decreasing the inert gas pressure to the solvent reservoir
and sample injection module (SIM).
G. A pressure gauge (labeled
PG-02
) for monitoring the inert
gas pressure delivered to the solvent reservoir and SIM.
H. A valve (labeled
MV-03
) for turning the flow of compressed
inert gas to the SIM on or off (see Table 2), with an instant-
tight fitting for attaching tubing (H2).
I. A valve (labeled
MV-02
) for turning the flow of compressed
inert gas to the solvent reservoir on or off (see Table 2), with
an instant-tight fitting for attaching tubing (I2).
Valve Positions
Valve
Off/Vent
On/Pressurize
MV-01
PRR-01
BIOTAGE
®
FLASH 75/150
CHROMATOGRAPHY
AM-190 MANIFOLD
PRR-02
M
V
-01
PG-01
PG-02
M
V
-03
M
V
-02
N
2
N
2
N
2
A
B
PRR-01
BIOTAGE
®
FLASH 75/150
CHROMATOGRAPHY
AM-190 MANIFOLD
PRR-02
M
V
-01
PG-01
PG-02
M
V
-03
M
V
-02
N
2
N
2
N
2
A
B
MV-02 and
MV-03
PRR-01
BIOTAGE
®
FLASH 75/150
CHROMATOGRAPHY
AM-190 MANIFOLD
PRR-02
M
V
-01
PG-01
PG-02
M
V
-03
M
V
-02
N
2
N
2
N
2
A
B
PRR-01
BIOTAGE
®
FLASH 75/150
CHROMATOGRAPHY
AM-190 MANIFOLD
PRR-02
M
V
-01
PG-01
PG-02
M
V
-03
M
V
-02
N
2
N
2
N
2
A
B
Table 2. The positions for the three valves on the AM-190 manifold.
Figure 4
PRR-01
BIOTAGE
®
FLASH 75/150
CHROMATOGRAPHY
AM-190 MANIFOLD
PRR-02
M
V
-01
PG-01
PG-02
M
V
-03
M
V
-02
N
2
N
2
N
2
A
B
E
H
I
C
F
A
C2
B
D
G
E2
I2
H2
. The AM-190 manifold (bottom) regulates the flow of inert gas
into the radial compression module, solvent reservoir, and sample
injection module (SIM). The front panel of the AM-190 manifold (top) offers
easy-to-use switches to set all flows.
A = pressurized inert gas inlet, B = safety relief valve, C = regulator
for the gas pressure to the radial compression module, C2 = water
trap, D = gauge for the pressure delivered to the radial compression
module, E = compress/vent valve for the radial compression pressure,
E2 = instant-tight fitting for connecting a gas tube between the manifold
and the radial compression module, F = regulator for the gas pressure
to the solvent reservoir and sample injection module (SIM), G = gauge
for the inert gas pressure delivered to the solvent reservoir and SIM,
H = compress/vent valve for the solvent pressure, H2 = instant-tight
fitting for connecting a gas tube between the manifold and the solvent
reservoir, I = compress/vent valve for the SIM pressure, and I2 = instant-
tight fitting for connecting a gas tube between the manifold and the SIM.
Summary of Contents for Flash 150
Page 1: ...Biotage Flash 75 150 User Manual ...
Page 34: ...32 Biotage Flash 75 150 User Manual Biotage 2018 Notes Notes ...
Page 35: ......