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Biotage
®
Flash 75/150 User Manual | © Biotage 2018
Optimizing Flash Separations
The Relationship Between the
Size of a Column and the Sample
Load is Dependent on ΔCV
Table 8 shows recommended sample loads for different length
Flash 75 and 150 prepacked cartridges. In general, the more
difficult the separation (i.e. a low ΔCV), the less material can
be loaded onto the cartridge for a successful/high resolution
separation.
Column
Load
ΔCV = 0.1–1.9
Load
ΔCV = 2.0–3.9
Load
ΔCV = 4+
Flash 75M
<4 g
4–20 g
10-40 g
Flash 75L
<8 g
8–40 g
40–80 g
Flash 150M
<25 g
25–125 g
125-250 g
Flash 150L
<50 g
50–250 g
250–500 g
Table 8. Predicting sample loads for prepacked flash cartridges.
Summary of Contents for Flash 150
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