Berthold Crocodile miniWorkstation Operating Manual Download Page 1

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LB 925

Crocodile miniWorkstation

Operating Manual

84024BA2

Rev. No.: 03, 04/2020

Summary of Contents for Crocodile miniWorkstation

Page 1: ...LB 925 Crocodile miniWorkstation Operating Manual 84024BA2 Rev No 03 04 2020...

Page 2: ......

Page 3: ...H OR ARISING FROM THIS DOCUMENT INCLUDING YOUR USE OF IT This instrument is not designed or intended for use with installations or equipment in hazardous environments Servicing of the instrument must...

Page 4: ...onsignes de S curit 15 2 3 Sicherheitshinweise 18 2 4 Further instructions 21 3 Installation 23 3 1 Unpacking and Check of Shipment 23 3 2 Setup and Installation of Hardware 23 3 3 Installation of the...

Page 5: ...hecks 43 6 1 1 Washer 43 6 1 2 Dispenser 44 6 1 3 Shaker 44 6 1 4 Incubator 44 6 1 5 Reader 5 in one only 44 6 2 Quality control measurements 45 7 Cleaning and Maintenance 46 7 1 Important information...

Page 6: ...24BA2 Rev 03 04 2020 9 3 Accessories 63 10 Preparing Crocodile for Transport 64 11 Warranty and Technical Issues 65 11 1 Special spare parts 65 11 2 Warranty statement 65 11 3 Customer service 66 11 3...

Page 7: ...ERTHOLD TECHNOLOGIES GmbH Co KG Calmbacher Stra e 22 D 75323 Bad Wildbad Germany Sales and Customer Support Internet www berthold com en bioanalytic Phone 49 0 7081 177 0 Fax 49 0 7081 177 100 E Mail...

Page 8: ...waste Keep cover closed The cover must be closed before start of any operation 1 4 The Operating Manual 1 4 1 Validity of the operation manual The operating manual is valid from the delivery of the B...

Page 9: ...2 02 07 2018 Version identification according to BERTHOLD TECHNOLOGIES standard Style sheet changed Software protection system revised 84024BA2 03 04 2020 Revision of safety instructions maintenance d...

Page 10: ...he software Special note On screenshots Submenu or information of special importance 1 5 Intended Use The Crocodile miniWorkstation has been specifically designed to automate assay applications in 96...

Page 11: ...y clinical chemistry Food industry e g development and quality control in production Bioanalytical research e g at universities and pharmaceutical industry Binding assays e g for sample preparation wi...

Page 12: ...The operating instructions contain information and warnings to be observed by the user to ensure safe operation of the device If legal regulations exist for the installation and or use of sample measu...

Page 13: ...ponsibly to avoid injuries such as chemical burns or contamination and potential infection Explosive and flammable substances must not be used with the instrument When handling cleaning and decontamin...

Page 14: ...it cleaned by an authorized service center Prior to repair or maintenance the instrument must be cleaned and decontaminated to prevent service personnel from coming into contact with potentially bioha...

Page 15: ...nnement s r de l appareil S il existe des prescriptions l gales pour l installation et ou l utilisation d appareils de mesure d chantillons dans le pays d installation l installateur et l exploitant s...

Page 16: ...dans l enceinte du syst me d essai par exemple en ce qui concerne les performances et la plage de temp rature Porter l quipement de protection appropri comme un sarrau de laboratoire ou des gants de...

Page 17: ...diatement la fiche secteur N utilisez jamais l appareil si des composants internes ont pu tre en contact avec des liquides car cela pourrait provoquer un choc lectrique ou un incendie Faites nettoyer...

Page 18: ...befolgende Informationen und Warnhinweise die den sicheren Betrieb des Ger tes zu gew hrleisten Bestehen f r die Errichtung und oder den Betrieb von Probenmessger ten im Land der Installation gesetzl...

Page 19: ...ein Infektionsrisiko oder eine andere Art von andere Gef hrdung f r den Anwender darstellen Seien Sie auch vorsichtig beim Umgang mit Glasgef en Halten Sie immer die Sicherheitsma nahmen wie sie in de...

Page 20: ...els gekennzeichnet Falls Fl ssigkeit in das Innere des Ger tes gelangt sein k nnte sofort den Netzstecker ziehen Betreiben Sie das Instrument niemals wenn interne Komponenten m glicherweise mit Fl ssi...

Page 21: ...nd protected from direct sunlight and significant temperature fluctuations Storage temperature 5 40 C up to 75 humidity at 30 C non condensing Transport conditions 25 to 60 C up to 75 humidity non con...

Page 22: ...le contains electronic parts To prevent environmental pollution please dispose of the instrument and the corresponding accessories according to local legislation The electronic product must not be dis...

Page 23: ...ed for future shipment or service See chapter 10 for details NOTICE Should the instrument or instrument parts be damaged in any way please inform the shipping agent or the manufacturer immediately 3 2...

Page 24: ...ll the acrylic cover as shown in the drawing below using the two cover pins and fix them with two screws each at the right and left side of the cover Rotate cover pins anti clockwise while inserting H...

Page 25: ...iWorkstation to PC use the supplied USB cable and read additionally chapter 5 1 Software Installation 3 5 Operating conditions NOTICE Operation Temperature 10 35 C up to 75 humidity non condensing The...

Page 26: ...Waste bottle Washer manifold Dispenser Waste container Washer lines Microplate holder Reagent lines 4 1 Microplate holder All 96 well microplates in standard format can be run on the Crocodile miniWo...

Page 27: ...nser and washer lines Waste container CAUTION The liquids are exhausted automatically after each priming or washing step The waste line must be connected to the waste outlet marked with Waste at the i...

Page 28: ...spenser lines and tips are preinstalled and ready to use Four bottle holders are provided with the instrument Fix the vials with liquids to be dispensed to the bottle holders to prevent them from fall...

Page 29: ...C up to 55 C This function is controlled via software When working with these functions microplates with flat bottoms are strongly recommended to achieve optimum heat transfer The microplate can be he...

Page 30: ...620 nm There are eight independent photometric reading channels An additional channel is used for regulation of the lamp The plate holder with the microplate moves into the reader unit positioning th...

Page 31: ...al and has to be ordered separately The MikroWin software is protected by a USB dongle The description of the MikroWin module is not part of this manual 5 1 Software Installation and connection NOTICE...

Page 32: ...the required information in the predefined mail fax form or web registration form and forward it to BERTHOLD TECHNOLOGIES 7 Alternatively request the password via the BERTHOLD TECHNOLOGIES homepage P...

Page 33: ...the system for error free operation 1 Disable the Power Save Mode of your PC before using Crocodile Control Software 2 Connect the instrument to a PC via USB cable 3 Connect the instrument to the main...

Page 34: ...tions to program an assay according to your needs Assay Name Select copy or create a new assay Washer Dispenser Inlet Label Define buffer and reagent positions The information will be listed in the re...

Page 35: ...4 6 6 When a step in the List of Selected Steps is highlighted its parameters are shown in the sub window Step Parameter 7 Confirm all entries with OK to save the assay 5 4 1 Edit an incubation step...

Page 36: ...peed into the Shaker dialog box 2 For shaking inside the incubator tick the respective check box 3 Confirm your entries with OK 5 4 3 Edit a washing step Washing is one of the most important steps in...

Page 37: ...t the default setting of the Plate Adjustment for the Dispense step needs to be optimized to define the optimal horizontal position for the dispensing of wash buffer The Manifold Depth and the Plate A...

Page 38: ...are available Before use of a dispenser it has to be primed 1 Select a Reagent inlet and set the Volume 2 For priming tick the check box Prime 3 For dispensing select the columns if necessary By defau...

Page 39: ...d Reference Measurement two measurements in the Method drop down list box 2 Choose a filter from the list of available optical filters in the Measurement drop down list box 3 If you have selected Refe...

Page 40: ...system will not continue with the next assay step before the user clicks Continue which will temporarily replace the Abort box during an assay run If a Manual step with User Continue mode has been se...

Page 41: ...se do not select any wells here and confirm with OK The protocol will now be performed according to the assay setting 7 Press the button Start to start the assay Crocodile will perform the defined ste...

Page 42: ...elected in accordance to the needs of the assay performed Please read the kit insert for recommendations The whole system of Crocodile miniWorkstation assay PC software and evaluation method both PC b...

Page 43: ...rument specific optimized depth for your specific microplate for dispensing and aspiration prior to the check Use the Crocodile Control Software and set up a protocol with the following settings 1 Pri...

Page 44: ...dispensing then and subtract the empty weight to get the weight of the dispensed water 4 Divide this value by the number of dispensed wells to get the weight per well 5 Expected weight 100mg 2mg 2 per...

Page 45: ...ckage insert of the reagent kit or the standard laboratory protocol for the test to be conducted Obtain treat and store samples following the instructions and recommendations of the kit insert Recomme...

Page 46: ...tion verify additionally the recommendations in the package insert of your test kit Wear appropriate protective equipment such as laboratory coats or chemically resistant rubber cloves and to act care...

Page 47: ...Clean Empty Reagent Bottles Waste Container Replace liquid tubing Table 7 1 Maintenance schedule 7 2 Cleaning 7 2 1 Chemical resistance of materials IMPORTANT The surfaces all materials of the pump s...

Page 48: ...T Always remove reagent residues with a high volume of distilled water e g up to 10 fold volume of the reagent used for cleaning Cleaning of dispenser and the washer dispenser unit Use the Maintenance...

Page 49: ...increased volume 4 0 1N HCl 5 Distilled water increased volume 6 Empty and dry the lines by pumping air Never mix acid and base in the dispenser or washer unit or the waste container directly Always r...

Page 50: ...led water increased number of columns 4 empty and dry the lines by pumping air Cleaning with acid base in case of heavy pollution 1 Distilled water 2 0 1 N NaOH 3 Distilled water increased number of c...

Page 51: ...larly performed maintenance step 1 Switch off the instrument and disconnect it from the mains 2 Take the manifold carefully out of the fixture 3 Insert the cleaning wires from below to dislodge the cl...

Page 52: ...ose 7 3 Decontamination CAUTION In cases of biohazard spillage other kinds of pollution or before service or reshipment all accessible parts of the instrument must be decontaminated 1 Decontaminate al...

Page 53: ...afterwards 4 Follow the description for cleaning the internal waste container and the extraction hose but wipe out with 0 5 bleach multiple times and with distilled water afterwards Dry the waste con...

Page 54: ...efer to the Maintenance Schedule in chapter 7 1 Operate as follows 1 Empty the dispenser lines 2 Turn the instrument off and disconnect it from the mains 3 Open the drawer at the side of the instrumen...

Page 55: ...he dispenser 7 A Dispenser Tubing Set comes with the instrument and provides tubing for the dispenser lines Turn the fitting screw of the new line into the thread at the dispenser and finger tighten i...

Page 56: ...to get rid of the dirt in the needles Rinse the washer afterwards Washing seems to be not good enough Optimize increase the height of the dispense step Use the soak wash program Increase the delay ti...

Page 57: ...ough priming Check for leakages in the dispense path air bubbles in the tubing tubing is not correctly tightened Check if the dispense path is blocked flush the dispense path Clean the needle with the...

Page 58: ...check the temperature Adjust the Assay protocol Microbiological contamination of the washer system Clean and decontaminate the washer system according to the description in the chapter Maintenance If...

Page 59: ...eck pipetting technique and double check calculations Reagents poorly mixed not at room temperature before the assay starts Be sure that reagents are thoroughly mixed and at the right temperature befo...

Page 60: ...incubation time and sample volume for your system Read your assay insert for recommendations Wrong temperature used during assay performance Perform the performance check to ensure that the incubator...

Page 61: ...y non condensing in original cardboard box and free of liquids Operating Conditions 10 C 35 C up to 75 humidity non condensing The system can be operated up to 2000 m above NN Interface USB Tubing Che...

Page 62: ...2 0 3 0 OD Accuracy 0 01 OD or 2 5 whichever is greater Dispenser Type 4 independent precision pumps Volume range 10 2000 l in increments of 5 l Precision 2 CV at 100 l Accuracy 5 at 100 l Dead Volum...

Page 63: ...7 32 Bit and 64 Bit Windows 8 32 Bit and 64 Bit Windows 10 32 Bit and 64 Bit Additional Software MS Excel TM optional 9 2 External Power Supply Type Desktop power supply Input 100 240 VAC 10 50 60 Hz...

Page 64: ...g to the description in chapter 7 2 7 3 2 Clean and disconnect waste tubing 3 Turn Crocodile off and disconnect the power cable 4 Move microplate holder manually to the front position and fix it with...

Page 65: ...manship for a period of 12 months from the date of delivery ex works Bad Wildbad Berthold Technologies or its authorized representative will repair or replace at its option and free of charge any prod...

Page 66: ...ftware expertise contact the Technical Support group at Berthold Technologies GmbH Co KG Here are the contact data Berthold Technologies GmbH Co KG Technical Support Calmbacher Str 22 75323 Bad Wildba...

Page 67: ...hnologies for servicing purposes which is not properly decontaminated there will be a health risk for Berthold Technologies employees We therefore need your confirmation that the instrument was decont...

Page 68: ...rement chamber 30 Measurement Units 42 Microplate holder 26 Operating Conditions 25 Password request 32 Performance checks 43 Preface 7 Preparing for transport 64 Quality control 21 45 Reader 30 Retur...

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