Agilent Technologies G1323A, User Manual, Page: 130 / 170

130 1200 Series Handheld Control Module Users’s Guide
10 Running a Gradient Analysis
Observing the Chromatogram
9
Press the
Plot
button [
F6
] to show the chromatogram.
A typical gradient chromatogram for this analysis is shown in Figure 17 .
The exact profile of the chromatogram will depend on the column you have
used. Differences in retention times and areas of the peaks in your
chromatogram and the one shown in
Figure 17 might be a result of variations
in the concentration of the sample from batch to batch, the quality of the
solvents used and the column temperature.
If you compare this chromatogram with the one from Chapter 9 , “Running an
Isocratic Analysis” you will notice the solvent gradient has reduced the elution
time of the fourth peak in the chromatogram.
Figure 17 Gradient Analysis of Isocratic Standard
min
1 2 3 4
mAU
0
50
100
150
200
250
300
350
N O T E
You can rescale the plot using the Rescale button [ F7 ], or the cursor keys or you define the
plot window within the Setup.