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Figure 2
1 mL Plating Instructions - Dry Swabbing Method -
1
Label each 3M Quick Swab.
2
Twist and pull apart the bulb end
of the swab from the tube end of
the swab that contains the letheen
broth.
3
Hold the swab handle to make a
30° angle with the surface. Rub
the swab slowly and thoroughly
over the desired surface area.
Rub the swab three times over
this surface, reversing direction
between alternating strokes.
4
After sampling is complete,
securely insert the swab back into
the swab tube and transport to
the lab for inoculation. Plate the
letheen broth swab solution as
soon as possible.
5
Bend the red snap valve at a 45°
angle until you hear the valve
break. This allows the letheen
broth to flow into the tube end
and wet the swab.
6
Squeeze the bulb of the swab to
transfer all of the letheen broth
to the tube end of the swab.
To complete inoculation, continue with steps 7-9 (See Figure 1 above)
Multiple mL Plating Instructions:
Plating multiple bacteria tests using the 3M Quick Swab (Wet or Dry Method)
a. Complete Steps 1 through 6 (Figures 1 or 2 above) for either the Wet or Dry swabbing method.
b. Remove the 3M Quick Swab from the tube. Add 1-3 mL of sterile diluent to swab tube. Replace the swab in the tube.
c. To prepare the sample for inoculation, vigorously shake or vortex 3M Quick Swab for 10 seconds to release the bacteria
from the swab tip.
d. Wring out the contents of the swab tip by pressing and twisting the swab against the wall of the tube.
e. Use your thumb to bend the swab tube at a 90° angle at the highest mark that has a diluent above it. Pour off a 1mL
aliquot onto a 3M Petrifilm Plate. Repeat until the entire sample is used.
f. Follow current industry standards for disposal.
The entire contents of 3M Quick Swab may also be added to any sterile dilution blank. Pipette 1mL aliquots from the
dilution blank onto 3M Petrifilm Plates.
If used with 3M Petrifilm Plates:
Refer to the appropriate 3M Petrifilm Plate “Product Instructions.”
If you have questions about specific applications or procedures, please visit our website at www.3M.com/foodsafety or
contact your local 3M representative or distributor.
NOTE:
As with any swab sampling method, the best microbial practice is to re-sanitize the swabbed area after the swab
sample has been taken.
