OPERATING INSTRUCTIONS
Lattice Lightsheet 7
System Operation
ZEISS
04/2021 V_01
000000-2324-960
79
13.
Zoom on some structures in the sample and use
aberration control (= Alvarez plates) to maximize
sharpness & contrast.
14.
Optionally save these settings for later use.
Once the system is aligned only the stage is
moved!
Lightsheet and detection objective are fixed –
focusing is done by the stage (moving the
sample)!
If you move to different sample areas and the
image gets blurry or defocused, don’t touch
Lightsheet / Aberration controls! Instead, just
use the z-wheel to bring back the fluorescent
line to the center of the image.
Fig. 66
Lightsheet aberration control. Upper
panel not corrected; lower panel
corrected using Aberration Control