Figure 1–2: Comparison of chromatographic separations using 5.0-µm and 1.7-µm
particles
The figure above compares two separations, one using HPLC with a column packed with 5 µm
particles, and the other using UPLC with a column packed with 1.7 µm particles. The
improvements in both resolution and the speed of analysis are apparent in the UPLC
chromatogram. Each separation was performed on a 2.1 × 50 mm column. Chromatographic
conditions for the separations were identical, except for the flow rate, which was scaled based on
particle size.
1.2 Features of the ACQUITY UPLC H-Class system
The ACQUITY UPLC H-Class system combines the speed and performance of UPLC with the
ability to run HPLC separations. This combination provides many benefits, including these:
• High-pressure, small-particle chromatography allowing faster, higher-resolution analyses,
compared with conventional HPLC
• Low solvent consumption (significantly less than conventional HPLC)
• Flexibility in solvent mixing by using a quaternary solvent manager
• A flow-through-needle sample manager
August 8, 2016, 715005049 Rev. C
Page 16
