Figure 2–1: Method transfer from HPLC to UPLC
When transferring methods from one system to another, you must define and characterize the
original method well. Doing so includes noting information about column dimensions, dwell
volumes, configurations, injection volumes, analyte molecular weights, and gradient profiles. You
must calculate various measurements of both systems, using the same method, to ensure a
successful transfer. Using the Waters ACQUITY UPLC Columns Calculator ensures the best
results for transferring the LC method from HPLC to UPLC or UPLC to HPLC.
August 8, 2016, 715005049 Rev. C
Page 43
