C.2.2 SM-FTN and bioSM-FTN performance specifications
Item
Specification
Injection volume
range
• With the standard loop fitted, 0.1 to 10.0 µL, in 0.1 µL increments.
• With one of the optional extension loops fitted, as much as the volume
of the extension loop (50, 100, 250, or 1000 µL).
Accuracy
(aspiration)
±0.2 µL, measured by fluid weight removed from vial by means of 10-µL
injections, averaged over 20 injections, using a 100-µL syringe.
Linearity
>0.999 (standard needle)
Test conditions:
• Chromatography: isocratic
• Mobile phase: 10:90 acetonitrile/water
• Flow rate: 0.6 mL/min
• Needle volume: 1 to 70%
• Sample mix: caffeine 0.03 mg/mL (0.2 to 10.0 µL, with 15 µL needle
installed, no extension loop)
• Column: ACQUITY UPLC BEH C
18
, 1.7 µm, 2.1 × 50 mm
• Detector: UV, 273-nm wavelength
• Column temperature: 40 °C ±0.3 °C
Precision
<1% area RSD, 0.2 to 1.9 µL (0.25 to 0.50 mg/mL caffeine),
<0.5% area RSD, 2.0 to 10.0 µL (0.03 mg/mL caffeine)
Test conditions:
• Chromatography: isocratic
• Replicates: 6
• Mobile phase: 10:90 acetonitrile/water
• Flow rate: 0.6 mL/min
• Column: ACQUITY UPLC BEH C
18
, 1.7 µm, 2.1 × 50 mm
• Detector: UV, 273-nm wavelength
• Column temperature: 40 °C ±0.3 °C
August 8, 2016, 715005049 Rev. C
Page 107
