Table B–4: Wavelength cutoffs for different mobile phases (continued)
Mobile phase
UV cutoff (nm)
Polyoxyethylene (35) lauryl ether (BRIJ 35), 0.1%
190
3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate) (CHAPS) 0.1% 215
Diammonium phosphate, 50 mM
205
(Ethylenedinitrilo) tetraacetic acid disodium salt (disodium EDTA), 1 mM
190
4-(2-hydroxethyl)-1-piperazineet hanesulfonic acid (HEPES), 10 mM, pH 7.6
225
Hydrochloric acid, 0.1%
190
Morpholinoethanesulfonic acid (MES), 10 mM, pH 6.0
215
Potassium phosphate, monobasic, 10 mM
190
Potassium phosphate, dibasic, 10 mM
190
Sodium acetate, 10 mM
205
Sodium chloride, 1 M
207
Sodium citrate, 10 mM
225
Sodium dodecyl sulfate
190
Sodium formate, 10 mM
200
Triethylamine, 1%
235
Trifluoracetic acid, 0.1%
190
TRIS HCl, 20 mM, pH 7.0
202
TRIS HCl, 20 mM, pH 8.0
212
Triton X-100, 0.1%
240
Waters PIC Reagent A, 1 vial/liter
200
Waters PIC Reagent B-6, 1 vial/liter
225
Waters PIC Reagent B-6, low UV, 1 vial/liter
190
Waters PIC Reagent D-4, 1 vial/liter
190
August 8, 2016, 715005049 Rev. C
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