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85-102113-00:1

Warning Symbol

Warning Description

Hazardous voltages are present inside
instrument. Disconnect from main power
before removing screw-attached panels.

Hazardous chemicals may be present. Avoid
contact, especially when replenishing
reservoirs. Use proper eye and skin
protection.

Very hot or cryogenically cold surfaces may
be exposed. Use proper skin protection.

Eye damage could occur either from flying
particles, chemicals, or UV radiation. Use
proper eye and face protection.

The potential for fire may be present. Follow
manual instructions for safe operation.

The potential for explosion may exist
because of type of gas or liquid used.

Ionizing radiation source is present. Follow
manual instructions for safe operation.

Keep hands and fingers away.

WARNING:
HEAVY WEIGHT

A heavy object is present. Avoid back strain
or injury by following all precautions for lifting
heavy objects.

Содержание ProStar 335

Страница 1: ...ngvale Rd Mulgrave Victoria 3170 Australia Varian Inc 2004 Printed in Australia 8510211300 Rev 1 ProStar 335 Photodiode Array Detector Operation Manual Overvoltage category II Pollution degree 2 Safet...

Страница 2: ...Varian Inc Internet home page can be found at www varianinc com Varian Australia Pty Ltd is the owner of copyright on this document and any associated software Under law the written permission of Vari...

Страница 3: ...2001 EN61000 4 6 1996 A1 2001 BSEN61000 4 11 1994 Equipment Model Number ProStar 335 LC Detector Series Authorized Representative in the EU Name G A Wassink Company Name Varian BV Signed Address Hercu...

Страница 4: ......

Страница 5: ...nization signals 57 Desktop PC communications 61 Networks 62 Wiring guide 67 Hydraulic connections 68 Installing a flowcell 69 Detector outlet back pressure restrictor 73 Installing the door 74 Instal...

Страница 6: ...he new lamp 96 Replacing the visible lamp 97 Installing the new lamp 99 Calibrating the lamps 100 Fuses 103 Cleaning the instrument 105 Diagnostics 106 Troubleshooting 107 Excessive noise and or drift...

Страница 7: ...in accordance with this instruction manual and any additional information which may be provided by Varian Address any questions regarding the safe and proper use of your equipment to your local Varian...

Страница 8: ...d Use proper skin protection Eye damage could occur either from flying particles chemicals or UV radiation Use proper eye and face protection The potential for fire may be present Follow manual instru...

Страница 9: ...that warning label Information symbols The following symbols appear on the ProStar 335 detector to provide you with additional information I Mains power on Mains power off Single phase alternating cu...

Страница 10: ...o carry the European Union CE Mark This equipment may be susceptible to radiation interference levels or frequencies which are not within the tested limits WARNING This instrument is designed for chro...

Страница 11: ...tion between the metal base of the detector and the 3 pin earth grounded receptacle Consult the manuals or product labels supplied with your PC for the relevant grounding requirements Replace blown fu...

Страница 12: ...uct outlet Locate duct intakes such that their vibration or air movement does not affect detector operation Check periodically for proper operation of the duct Ensure proper ventilation in lab area Ra...

Страница 13: ...ressure Read and adhere to all NOTES CAUTIONS and WARNINGS in the manual Flash Chromatography The operator should be familiar with the physico chemical properties of the components of the mobile phase...

Страница 14: ...on of ozone does not exceed the maximum permissible level All venting must be to outside air never within the building Federal Communications Commission advisory The following is a Federal Communicati...

Страница 15: ...aration of Conformity This Declaration is the legal declaration by Varian that the product complies with the Directives and also shows the standards to which the product was tested to demonstrate comp...

Страница 16: ...2 800 Brazil and Latin America S S o Paulo Tel 55 11 820 0444 Canada Mississauga Ontario Tel 800 387 2216 China Beijing Tel 86 106209 1727 Europe Middelburg The Netherlands Tel 31 118 671 000 France L...

Страница 17: ...CION como se indica m s abajo Es importante que utilice el instrumento de acuerdo con este Manual de Operaci n y cualquier otra informaci n que le proporcione Varian Remita a la Oficina Local de Varia...

Страница 18: ...e protecci n para la piel Las part culas vol tiles productos qu micos o radiaci n UV pueden causar da os en los ojos Usar las debidas protecciones para la cara y los ojos ATENCI N PELIGRO DE FUEGO Pel...

Страница 19: ...especial de desechos y no a trav s del sistema convencional NOTA Este instrumento ha sido testado bajo las normas de la Directiva EMC seg n requerimientos de la Marca CE de la Uni n Europea Por lo ta...

Страница 20: ...e los valores de las l neas de electricidad se ajustan a los valores para los que el Instrumento ha sido preparado Botellas de Gas Comprimido Guarde y maneje las botellas de gas con cuidado y de acuer...

Страница 21: ...stema Asegurese de una correcta ventilaci n del laboratorio Detectores con fuentes radioctivas Lea con cuidado y cumpla todas las NOTAS PRECAUCION y ATENCION del Manual del Detector Ni63 ECD Realice l...

Страница 22: ...ash El operador debe familiarizarse con las propiedades f sico qu micas de los componentes de la fase m vil Alejar los disolventes del contacto directo con los tubos de poliuretano ya que ciertos diso...

Страница 23: ...ado Los recambios durante esos cinco a os estar n disponibles pero siempre bajo el sistema Seg n disponibilidad Los Recambios est n definidos como todas aquellas partes individuales mec nicas el ctric...

Страница 24: ...delburg The Netherlands Tel 31 118 671 000 France Les Ulis C dex Tel 33 1 6986 3838 Germany Darmstadt Tel 49 6151 7030 India Mumbai Tel 91 22 857 0787 88 89 Italy Torino Tel 39 011 997 9111 Japan Toky...

Страница 25: ...ner ce mat riel conform ment aux instructions du pr sent manuel et toute autre information manant de Varian S adresser au bureau r gional Varian pour toute question relative la s curit ou l utilisatio...

Страница 26: ...otection ad quates pour la peau Les dommages caus es aux yeux sont de deux natures diff rentes jet de particules et de produits chimiques ou radiations UV Utiliser des protections du visage et des yeu...

Страница 27: ...but Les liminer conform ment un programme agr d limination des d chets et non via les gouts municipaux NOTE Ce mat riel a t test conform ment aux dispositions de la directive CME afin de pouvoir porte...

Страница 28: ...n de secteur correspondent la tension de fonctionnement du mat riel Bouteilles gaz comprim Ranger et manipuler les bouteilles gaz comprim avec pr caution et conform ment aux normes de s curit Fixer le...

Страница 29: ...V rifier p riodiquement l tat du conduit S assurer que le laboratoire est correctement ventil D tecteurs source radioactive Se conformer au manuel d utilisation de l ECD Ni63 en particulier ses NOTES...

Страница 30: ...ET AVERTISSEMENTS du manuel d utilisation Chromatographie Flash L utilisateur aura la connaissance des propri t s physico chimiques des constituants de la phase mobile Eviter le contact direct des sol...

Страница 31: ...i ces de rechange ne sont fournies au terme de cette p riode de cinq 5 ans que suivant les disponibilit s Il faut entendre par pi ces de rechange les pi ces individuelles lectriques ou m caniques susc...

Страница 32: ...86 106209 1727 Europe Middelburg The Netherlands Tel 31 118 671 000 France Les Ulis C dex Tel 33 1 6986 3838 Germany Darmstadt Tel 49 6151 7030 India Mumbai Tel 91 22 857 0787 88 89 Italy Torino Tel 3...

Страница 33: ...nto venga utilizzato rispettando le istruzioni fornite in questo manuale o che verranno fornite successivamente dalla Varian Per ogni eventuale chiarimento sull uso o sulla sicurezza si prega di conta...

Страница 34: ...calde o raffreddate criogenicamente Usare protezioni opportune per la pelle Particelle volanti agenti chimici o radiazioni UV possono danneggiare gli occhi Vanno quindi utilizzate le opportune protez...

Страница 35: ...di scarico AVVERTENZA Questo strumento stato testato secondo le Direttive EMC allo scopo di poter utilizzare il Marchio CE della Comunit Europea Questo strumento pu essere suscettibile a radiazioni in...

Страница 36: ...llo stesso tipo e con le stesse caratteristiche Assicurarsi che il voltaggio del pannello di alimentazione corrisponda a quello dello strumento da collegare Bombole dei Gas Occorre prestare molta atte...

Страница 37: ...iche per garantire un funzionamento corretto Garantire una buona ventilazione nel laboratorio Rivelatori a Sorgente Radioattiva Leggere e rispettare tutte gli avvisi di NOTA CAUTELA e ATTENZIONE ripor...

Страница 38: ...re tutti gli avvisi di NOTA CAUTELA e ATTENZIONE Cromatografia Flash L operatore deve conoscere le propriet fisico chimiche delle componenti della fase mobile I solventi non vanno messi in contatto di...

Страница 39: ...a serie Le parti di ricambio saranno disponibili anche dopo questo periodo di cinque 5 anni ma solo in base alla disponibilit delle stesse Per parti di ricambio si intendono i componenti elettrici e m...

Страница 40: ...209 1727 Europe Middelburg The Netherlands Tel 31 118 671 000 France Les Ulis C dex Tel 33 1 6986 3838 Germany Darmstadt Tel 49 6151 7030 India Mumbai Tel 91 22 857 0787 88 89 Italy Torino Tel 39 011...

Страница 41: ...Es ist wichtig da Sie Ihr Ger t in bereinstimmung mit dieser Arbeitsanleitung und allen m glichen zus tzlichen Informationen von Varian betreiben Alle Fragen bez glich Sicherheit und Handhabung Ihres...

Страница 42: ...gelegt sein Benutzen Sie einen wirksamen Hautschutz Herumfliegende Partikel Chemikalien oder UV Strahlung k nnen Augensch den verursachen Tragen Sie deshalb einen geeigneten Schutz f r Augen und Gesic...

Страница 43: ...eregeltes Entsorgungsprogramm und nicht ber die ffentlichen Abwasserleitungen HINWEIS Dies Instrument wurde nach den zutreffenden Vorschriften der EMC Direktive getestet die zum F hren des CE Zeichens...

Страница 44: ...len und die Netzspannung den gleichen Wert haben f r den das Instrument verdrahtet ist Gasdruckflaschen Lagern und handhaben Sie komprimierte Gase vorsichtig und in strikter Einhaltung der Sicherheits...

Страница 45: ...sf hrung Sorgen Sie f r gute Entl ftung im Laborbereich Radioaktive Detektoren Lesen Sie sorgf ltig und befolgen Sie alle HINWEISE ACHTUNGEN und WARNUNGEN im Ni63 ECD Handbuch F hren Sie die Tests f r...

Страница 46: ...oirverkleidung geschlossen wenn die Reservoirs unter Druck stehen Lesen Sie und befolgen Sie alle HINWEISE ACHTUNGEN und WARNUNGEN im Handbuch Blitzlicht Chromatographie Der Bediener sollte mit den ph...

Страница 47: ...gsten Zubeh re f r einen Zeitraum von f nf 5 Jahren nach dem Fertigungsauslauf dieser Ger teserie verf gbar zu haben Nach diesem Zeitraum von f nf 5 Jahren k nnen Ersatzteile auf der Basis solange vor...

Страница 48: ...Middelburg The Netherlands Tel 31 118 671 000 France Les Ulis C dex Tel 33 1 6986 3838 Germany Darmstadt Tel 49 6151 7030 India Mumbai Tel 91 22 857 0787 88 89 Italy Torino Tel 39 011 997 9111 Japan...

Страница 49: ...velengths The detector has a deuterium UV lamp and a quartz iodide lamp is available to provide better sensitivity for the visible and NIR wavelengths The ProStar 335 detector is interfaced to the Sta...

Страница 50: ...e ProStar 335 detector are Stackable module Interchangeability of flowcells easily performed by the operator Simple lamp replacement procedure performed by the operator Figure 1 The Varian ProStar 335...

Страница 51: ...ure restrictor and to waste All power and signal cable connections are located on the rear panel of the module All hydraulic connections are made outside the covered area of the module and do not requ...

Страница 52: ...ce Unpacking 1 Ensure you have enough room on the bench to put the ProStar 335 detector once it is removed from the box 2 Carefully unpack the unit from the shipping carton and place it on the bench T...

Страница 53: ...rt number 02 101946 00 ProStar 335 detector door part number 01 07288 00 Door cap part number 09 102066 00 ProStar 335 CD ROM part number 85 102114 00 Country kit containing fuses and power cable part...

Страница 54: ...lines the distance between the column outlet and the flowcell inlet should be kept to a minimum For best performance the detector should be located on a clean sturdy vibration free bench in an area fr...

Страница 55: ...dules the usual position is above the pump or pumps and below the AutoSampler as shown in Figure 2 Figure 2 ProStar 335 in a stack with a ProStar Pump and AutoSampler If the ProStar 335 is not the onl...

Страница 56: ...ections are made on the rear panel of the ProStar 335 see Figure 3 The connectors on the rear panel support communication configurations to a wide range of Varian modules and non Varian devices such a...

Страница 57: ...nc signal 9 pin female D shell connector used with synchronization signal cable AC power The ProStar 335 detector may be connected to any voltage in the range 100 240 VAC 10 50 60 Hz 1 Hz without modi...

Страница 58: ...in the liquid chromatography system should be connected to the same power source using a properly grounded third wire to earth multiple outlet power strip Power strips for operation at 125 VAC 50 60 H...

Страница 59: ...ptional analog output cable part number 01 107442 00 into the J14 receptacle Pin designations are shown below The output levels of the analog output are described in the Appendix Figure 4 Pin designat...

Страница 60: ...the J4 receptacle Pin designations are shown below Figure 5 Pin designation for J4 There are four general purpose output relays and one dedicated Peak relay Each output uses a DIP relay that is capab...

Страница 61: ...relay can be activated from any of the following sources only one source can be active at any one time Time Slice event Once time slice has been turned on it will provide a periodic activation of the...

Страница 62: ...part number 01 107438 00 and a cable used to attach to the terminal strip part number 01 107441 00 With the 3 pin connectors the contact closure is between pins 1 and 2 of the plugs Pin 3 is connected...

Страница 63: ...ate in a HPLC system These signals are important for controlling timing and synchronization of the detector with the other devices in the system Synchronization signals are closely tied to the detecto...

Страница 64: ...rity of the signal wire connections The positive output signal must be connected to the positive input signal and the negative output to the negative input The color coding and physical design of the...

Страница 65: ...This polarized output signal will be activated for 600 milliseconds when the ProStar 335 detector starts a time program Fault In This polarized edge triggered input signal informs the ProStar 335 det...

Страница 66: ...into the P9 and J10 connectors and connects to a terminal strip on the adapter board This terminal strip accepts bare wire leads from cables connecting other devices These cables may originate from th...

Страница 67: ...h cable can be purchased either locally or from Varian part number 01 107233 00 Most PCs come pre configured with an Ethernet connection which is usually built into the motherboard or with an Ethernet...

Страница 68: ...evices to communicate with each other Communications over the Internet and the World Wide Web use TCP IP Since the ProStar 335 uses TCP IP for communication the Workstation PC and the ProStar 335 need...

Страница 69: ...BOOTP Server for IP address assignment Where to begin Before beginning the configuration process you should know whether you will be attaching your Workstation and ProStar 335 detector to a company n...

Страница 70: ...t might be necessary to remove the Ethernet Board from the computer to remove the incorrect Ethernet drivers You must configure your computer s network settings to allow communication with the ProStar...

Страница 71: ...o 6 in the previous procedure 1 Click Internet Protocol TCP IP in the network component list and then click the Properties button The TCP IP Properties dialog box is displayed 2 Select Specify an IP a...

Страница 72: ...r may instruct you to obtain an IP address automatically by selecting Obtain an IP address automatically For the appropriate address contact your Network Administrator or whoever assigns IP addresses...

Страница 73: ...INSTALLATION ProStar 335 Photodiode Array Detector 67 Wiring guide The following figure shows the wiring in a typical HPLC system Figure 10 Wiring guide...

Страница 74: ...uartz optics cell window Your chosen flowcell type is packed internally in the detector The four flowcell types are outlined in Table 2 Table 2 Compatible flowcells Flowcell type Flowcell P N Pathleng...

Страница 75: ...bing as shown in Figure 11 Figure 11 PEEK tube with ferrule and nut fitted Tubing connections are PEEK type except for the Super Prep flowcell which uses Tefzel tubing 0 125 x 0 062 Table 3 Compatible...

Страница 76: ...rew the two tubes into the body of the flowcell Be careful not to cross thread the connector into the flowcell body Do not over tighten as this will damage the threads Each flowcell has an inlet and o...

Страница 77: ...Detector 71 3 Remove the panel on the front right side of the detector by unscrewing the screw in the top left corner of the panel to access the flowcell compartment refer to Figure 13 Figure 13 Remo...

Страница 78: ...t the flowcell is inserted correctly The internal optical components of the flowcell are an integral part of the detector s optical system If the flowcell is not fitted correctly it will have an adver...

Страница 79: ...baseline from forming or being trapped in the flowcell Note that the arrow stamped on the restrictor body must point away from the flowcell outlet port and toward the waste receiver The threaded plast...

Страница 80: ...e door cap should be installed before installing the door on the module If the ProStar 335 detector is not at the top of the stack do not install the cap and proceed to the instructions describing doo...

Страница 81: ...door lip Be sure to keep the door edges and cap edges flush refer to Figure 15 Installing and removing the door To install the door 1 Insert the top hinge pin into the top hinge 2 Gently press down o...

Страница 82: ...76 85 102113 00 1 To remove the door 1 Gently push down on the door and slide the lower hinge pin out of the lower hinge 2 Lift and slide out the top of the door...

Страница 83: ...n Power button and indicator lights The power button and indicator lights are located on the front of the Varian ProStar 335 detector refer to Figure 17 Figure 17 Power button and indicator lights on...

Страница 84: ...the front of the ProStar 335 detector change color to indicate its current status The table below defines each status Table 4 ProStar 335 LED indicator lights LED Status Color Power Power up Initiali...

Страница 85: ...al components are pre aligned and sealed and must not be readjusted under any circumstance Optical path The optical path of the ProStar 335 detector is as follows for a graphical representation see Fi...

Страница 86: ...diffraction grating The dispersed light hits the exit collimating mirror before being focused onto the dual photodiode detectors by mirrors N4 and N5 Figure 18 Optical ray trace diagram UV lamp Visibl...

Страница 87: ...rned on by the Varian Star or Galaxie Chromatography Workstation the detector goes through its initialization sequence It can be turned off and on through the Workstation The light generated by the la...

Страница 88: ...tense When used simultaneously with the UV lamp the light throughput is largely increased in this region The visible lamp mounts on top of the optics module and is held in place by two screws These ar...

Страница 89: ...g is achieved by quartz optics lenses internally fitted to the flowcell Figure 21 A typical flowcell 9 x 1 Arrows show the liquid flow path Flowcells are made of titanium and have a maximum working pr...

Страница 90: ...ng is used to disperse the light of incidence into a continuous spectrum It provides an approximate linear dispersion and resolution across the entire UV Vis spectrum Diffraction grating details Grati...

Страница 91: ...Array Detector 85 Control from the Workstation All control of the ProStar 335 detector is done through the Varian Star or Galaxie Chromatography Workstation software Please refer to those driver manu...

Страница 92: ...86 85 102113 00 1 This page is intentionally left blank...

Страница 93: ...ie driver manuals respectively Starting up Starting up involves turning the power on and turning on the lamp Turning the power on WARNING EXPLOSION HAZARD There is a danger of ignition when using flam...

Страница 94: ...gh a series of electronic diagnostic checks If your detector fails to power up when the power switch is turned on check that the power cord is properly connected and check for power at the wall recept...

Страница 95: ...replacement will be necessary if a flowcell of different pathlength is desired or if cleaning procedures do not satisfactorily clean the cell Lamp replacement is required when the lamp output deterio...

Страница 96: ...roughly to remove any trace of organic solvents 2 After the system has been rinsed with water pump 25 phosphoric acid through the flowcell This acid is prepared by diluting one part of concentrated ph...

Страница 97: ...owcell and carefully blow any dust or contamination from the external windows 2 Replace the flowcell ensuring that the sample and reference beam windows are not obstructed For full details on removing...

Страница 98: ...tallation of the new one WARNING To avoid electric shock and eye damage turn the main power switch to OFF and disconnect the power cord before starting the lamp replacement procedure WARNING Let the l...

Страница 99: ...35 Photodiode Array Detector 93 1 Remove the door see page 76 2 Remove the front panel by unscrewing the captive screw in the top left corner of the panel refer to Figure 22 Figure 22 Removing the Pro...

Страница 100: ...102113 00 1 3 Squeeze the small latch on the 3 way connector and pull the connector out refer to Figure 23 and Figure 24 Figure 23 Removing the lamp 3 way connector Figure 24 Lamp 3 way connector rem...

Страница 101: ...tector 95 4 Undo the first captive screw refer to Figure 25 Figure 25 Undoing the first lamp screw NOTE Be sure to retain the screw for re installation 5 Undo the second captive screw refer to Figure...

Страница 102: ...eplacing the lamp to prevent any oil or other material from getting on the lamp glass envelope It is important that you never touch the glass on the lamp with your bare hands as this can cause the lam...

Страница 103: ...equires no alignment procedures after it has been installed Follow the steps given below for removal of the old lamp and installation of the new one WARNING Turn off the lamp and let the lamp housing...

Страница 104: ...olding screw refer to Figure 29 Figure 29 Undoing the visible lamp screw 4 Holding the screw remove the visible lamp housing refer to Figure 30 This will then expose the lamp which will still be conne...

Страница 105: ...lly pull the lamp towards you 6 Remove the lamp and discard No part of the old assembly can be re used Installing the new lamp CAUTION Do not touch the visible lamp glass with your fingers always use...

Страница 106: ...from the flowcell The back pressure restrictor provides approximately 40 psi back pressure on the flowcell This prevents outgassing and bubbles from forming or being trapped in the flowcell which can...

Страница 107: ...p calibration in the Star Chromatography Workstation software ensure the detector has the conditions outlined above then 1 Under Run Status click the Options button The Options dialog box refer to Fig...

Страница 108: ...3 Ensure that all the conditions outlined in the Calibrate Lamps dialog box are satisfied then click Yes to proceed The Calibrate Lamps Confirmation dialog box refer to Figure 33 will appear Figure 3...

Страница 109: ...avy or L low and the voltage rating y volts This code must correspond to the code appearing next to the fuse holders WARNING To prevent reduced safety protection or unwanted fusing ALWAYS ensure that...

Страница 110: ...104 85 102113 00 1 Figure 34 Mains input connector and fuse compartment Figure 35 Lifting the fuse compartment cap Figure 36 Sliding the fuse compartment out Mains input connector Fuse compartment...

Страница 111: ...ins input connector and then lower the cap 5 Reconnect the instrument to the mains power supply NOTE If a fuse blows repeatedly it may indicate other problems with the instrument A service call may be...

Страница 112: ...is detected the error is displayed in the software Extended diagnostics tests are available using the ProStar 335 PolyVerify software Extended diagnostics are those test procedures that may pinpoint...

Страница 113: ...the detector wavelength to 240 nm and a response time of 2 seconds 3 Use the ProStar 335 PolyVerify application to perform diagnostic tests to determine the source of the problem For information on us...

Страница 114: ...108 85 102113 00 1 This page is intentionally left blank...

Страница 115: ...190 950 nm Flowcell Optional 9 x 0 9 x 1 4 x 0 4 x 0 15 Pressure 1000 psi maximum on all flowcells Response time 0 05 0 5 1 0 and 2 0 sec Spectral bandwidth 1 2 4 8 or 16 nm Recorder output 1 V FS Da...

Страница 116: ...be replaced only by Varian authorized personnel Fuse information on the rear of the instrument is the most up to date External connections Mains inlet coupler 3 2 A 120 250 VAC 50 60 Hz IEC type Mains...

Страница 117: ...16 bit converter with an output range of 0 1 V FS with 11 20 over range This gives an analog output resolution at 1 AU Volt 1V 131 65535 20 uV 20 uAU The Full Scale ranges are Full Scale AU Resolution...

Страница 118: ...ion 130 VA max Environment Your instrument is designed for indoor use only It is suitable for the categories stated on the front of this manual Operation within specifications from 10 to 35 C without...

Страница 119: ...cable for Relay interface board one Relay contact per cable 0110744100 Interconnect cable open ended for adaptor board one signal per cable 0110744200 Assy loom LC analog signal cable 325 335 02101818...

Страница 120: ...114 85 102113 00 1 This page is intentionally left blank...

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