6 Programs
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The thermolock is activated. After sterilization, pressure is reduced step by step in
very small increments. There is no danger of overboiling. The vessels will cool in a
controlled manner. The sterilizing chamber is pre-aerated between 100 °C and 90
°C. However, no vacuum is created, so that the culture media will not boil in this
temperature range. The liquid loss by evaporation is thus reduced considerably.
After cooling to a reference temperature (if the media temperature control is
present: media temperature) of 90 °C, the program cycle is completed.
With Type RM fast recooling system expansion option:
Ö
Liquids RM
For minimizing batch times when sterilizing liquids or culture media in open or
slightly closed vessels.
Cooling water flows through the sterilizing chamber in a separate circuit. The
sterilized items are quickly and carefully cooled to a reference temperature (if
media temperature control is present: media temperature) of 90 °C.
6.2 Setting the sterilizing temperature
The selected set temperature (in °C) is shown on the cycle display (16).
The total available temperature range will depend on the operating data of your
sterilizer. The following range is valid for Germany
6))
:
Type 25T:
98 °C to 135 °C.
We recommend the following set temperatures for sterilization and disinfection:
98 °C
for disinfection; for steaming and dissolving agar-agar
103 °C
for thermally instable media
121 °C
for culture media, solutions and other liquids, as well as solids
such as glassware or instruments
134 °C
for destructive sterilization
You can set the set temperature on the control panel (5):
♦
Press the selected program key (18) repeatedly until the sterilizing
temperature appears in the cycle display (Figure 7).
Cycle
Steril. Temp.
121 C
°
Figure 7: Sterilizing temperature display
♦
Increase or decrease the sterilizing temperature using the
▲
key (14) or
the
▼
key (15).
The set temperature will increase or decrease in one-degree increments
with each press of the
▲
key (14) or the
▼
key (15).
6)
*
In other countries other temperature ranges for setting may be available.
