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Chapter 13: Glossary and Abbreviations

Thermo Scientific iEMS Incubator / Shaker HT User Manual Rev. 2.4, Cat. no. 1506400A

Glossary and Abbreviations

EIA

Enzyme immunoassay.

European Norm.

European Union.

IEC

International Electrotechnical Commission.

Incubator

An apparatus for maintaining optimal conditions (temperature, humidity, etc.) for
growth and development.

Microplate

A rigid or framed polystyrene plate with microwells in different well formats (for
example, 6, 12, 24, 48, 96, 384, 864, etc. wells) for ease of use in performing
multiple tests through techniques such as EIA or ELISA.

Orbital shaking

The shaking of a microplate in a circular movement with a fixed orbit and adjustable
speed.

rpm

Revolutions per minute.

13.1

Keywords for web pages

drug discovery

microwell plate

EIA orbital

shaker

ELISA orbital

shaking

enzyme immunoassay

orbital shaking incubator

immunoassay plate
incubating separation technique(s)
incubator shaker
incubator shaker

shaking

liquid handling

solid phase assay(s)

microplate

Thermo Fisher Scientific

microplate incubator

Thermo Scientific

Microtiter plate

Содержание iEMS Incubator

Страница 1: ...service in house repair center WE BUY USED EQUIPMENT Sell your excess underutilized and idle used equipment We also offer credit for buy backs and trade ins www artisantg com WeBuyEquipment REMOTE IN...

Страница 2: ...Thermo Scientific iEMS Incubator Shaker HT User Manual Rev 2 4...

Страница 3: ......

Страница 4: ...Thermo Scientific iEMS Incubator Shaker HT User Manual Rev 2 4 Cat no 1506400A...

Страница 5: ...sher Scientific reserves the right to change its products and services at any time to incorporate technological developments This manual is subject to change without prior notice as part of a continuo...

Страница 6: ...ption 10 5 Installation 14 6 Operation 19 7 Maintenance 34 8 Troubleshooting Guide 39 9 Technical Specifications 44 10 Ordering Information 48 11 Warranty Certificate 49 12 Index 50 13 Glossary and Ab...

Страница 7: ...for damage during transport 15 5 2 4 Climatic requirements 15 5 2 5 Things to avoid 15 5 2 6 Technical prerequisites 16 5 3 Setups before you put the instrument into operation 16 5 3 1 Voltage selecto...

Страница 8: ...2 2 General 40 8 2 3 Temperature 41 8 2 4 Shaking 41 8 2 5 Operation 42 8 2 6 Maintenance 42 8 3 Service request protocol 43 9 Technical Specifications 44 9 1 General specifications 44 9 2 Incubator s...

Страница 9: ...ly 16 Fig 5 2 Selecting the correct voltage 17 Fig 6 1 Principle of operation 19 Fig 6 2 Microplate in the thermal microplate holder 24 Fig 6 3 Inserting the thermal microplate holder into a slot 25 F...

Страница 10: ...anufacture Consult Instructions for Use WEEE symbol This product is required to comply with the European Union s Waste Electrical Electronic Equipment WEEE Directive 2002 96 EC Warning markings used i...

Страница 11: ...ecautions Install the iEMS Incubator Shaker HT Carry out incubating and shaking procedures Perform basic maintenance procedures Troubleshoot the instrument performance Maintain the instrument The user...

Страница 12: ...d one incubation module with three 3 incubation slots All slots can be used for processing microplates individually within certain limitations A control LED above each slot indicates the status of tha...

Страница 13: ...per heater within the top of the module prevents condensation from forming within the microplate holder For effective mixing a powerful variable speed orbital shaker is incorporated Featuring an orbit...

Страница 14: ...v 2 4 Cat no 1506400A 11 4 2 iEMS Incubator Shaker HT rear view 1 Cooling air inlet 2 DIP switches not in use 3 Device link RS 485 not in use 4 Computer RS 232C device link RS 485 not in use 5 Cooling...

Страница 15: ...thermal microplate holders to the temperature set in processing protocol parameters shake Starts shaking with the speed and time set in processing protocol parameters start Starts operation defined by...

Страница 16: ...Adjustment screws 5 Handle A1 Position of the A1 well on the microplate Fig 4 5 Thermal microplate holder The thermal microplate holder is designed for holding the microplate during the incubation pro...

Страница 17: ...the iEMS Incubator Shaker HT instrument and accessories carefully with the arrows on the transport package pointing upwards The following notes and instructions are sent with the instrument and are i...

Страница 18: ...requirements When you set up your iEMS Incubator Shaker HT avoid sites of operation with excess dust vibrations strong magnetic fields direct sunlight draft excessive moisture or large temperature flu...

Страница 19: ...operation 5 3 1 Voltage selector The voltage selector fuse block assembly Fig 5 1 is located on the left hand side Fig 4 1 item 8 of the instrument Check that the selected voltage corresponds to the...

Страница 20: ...embly back into the power entry port housing Snap the assembly securely into place 5 Reinsert the power cable 5 3 2 Mains supply cable Warning Never operate your instrument from a power outlet that ha...

Страница 21: ...nt The firmware version is shown on the display Set the clock time 1 While in the READY state press C to select the setup menu 2 Select 1 for clock Press Enter 3 Program the new time hh mm ss and pres...

Страница 22: ...the required slots Section 6 5 Preheating and heating before starting the operation Section 6 4 3 Operation It is also possible to start shaking the slots without heating as a separate function Secti...

Страница 23: ...tocol see Section 6 5 Preheating and heating Operation Display Switch the power ON Note If the power is already on any shaking or heating must be turned off in the slot to be able to program a protoco...

Страница 24: ...the cursor For example for a shaking time of 20 seconds Press 00 00 20 Note If you want to interrupt programming press stop to return to the READY state Your changes will be saved Interval time is a...

Страница 25: ...er Value 0 1 2 3 4 5 Shake speed rpm 0 400 650 900 1150 1400 Caution Splashing may occur if you use shaking with wells that contain too much liquid for a given speed To avoid splashing and potential c...

Страница 26: ...he set temperature has not yet been reached Orange The slot has reached the set temperature and is ready for operation Red The slot is in use shaking and or incubating Green The processing protocol is...

Страница 27: ...te holder from its cover since it will come off Use the handle Fig 6 2 Caution Do not place the microplate holder into the instrument while the instrument is shaking This may damage the shaking mechan...

Страница 28: ...al operating position Caution Do not place the microplate holder in or remove it from the instrument while the instrument is shaking This may damage the shaking mechanism Fig 6 3 Inserting the thermal...

Страница 29: ...rocessing protocol The slot has reached the target temperature when the orange LED has stopped flashing and stays continuously orange Before you start the processing protocol ensure the temperature of...

Страница 30: ...ent is shaking This may damage the shaking mechanism 6 4 3 2 Checking the operation time During operation you can check the remaining incubation time by pressing the slot number For example Press 1 If...

Страница 31: ...cubation 6 4 3 5 Stopping the operation If you press a slot number and stop before the processing protocol time elapses the protocol stops and incubation stays on The LED will light up with a green co...

Страница 32: ...is green and you hear the audio signal The processing protocol is complete but the heating of the slot is still on If you wish to turn the heating off press the slot number 1 3 and then press stop Not...

Страница 33: ...control to a holder is lost or enters a runaway condition Microplate holders at this temperature will be hot when touched To help prevent loss of temperature control ensure the power contacts on the b...

Страница 34: ...e to another slot Before the temperature has reached the set value the LED flashes with an orange color After the temperature has reached the set value the orange LED lights up continuously Press temp...

Страница 35: ...te shaking without heating as a separate function by pressing the shake key Press shake Press the slot number 1 3 For example Press 1 To start shaking press start The shaking time and speed are the sa...

Страница 36: ...6 7 Verifying incubation and shaking parameters You can display the parameters programmed for each slot To display the programmed parameters press the appropriate slot number and then press Enter Con...

Страница 37: ...holder with clean low pressure compressed air or a cloth dampened with water or a mild detergent when necessary Caution Painted surfaces can be cleaned with most laboratory detergents Dilute the clean...

Страница 38: ...om the power input socket 2 Use a small flat screwdriver to pry the voltage selector fuse block assembly away from the power entry port housing and remove Fig 7 1 Fig 7 1 Opening the voltage selector...

Страница 39: ...instructions provided with the reagents used should be followed A decontamination procedure is only recommendable when infectious substances have been in direct contact with any part s of the instrume...

Страница 40: ...disposable gloves 2 Absorb the spill with disposable towels 3 Dampen a disposable towel with 2 glutaraldehyde containing decontaminating solution and clean the surfaces of the thermal microplate hold...

Страница 41: ...instrument prior to disposal See Section 7 4 and Appendix B on decontamination Follow laboratory and country specific procedures for biohazardous or radioactive waste dispoal Warning The used batterie...

Страница 42: ...ment Refer to Section 6 5 Module incubation temperature All slots within a module will be heated to the same temperature The slots within a module are kept at the temperature that is programmed into t...

Страница 43: ...ch liquid for a given speed To avoid splashing and potential cross contamination from well to well do not overfill wells for a given shaking speed setting When the well is filled to 50 of its nominal...

Страница 44: ...not prevent the processing protocol from starting if the slot is not at the target temperature You can view the actual slot temperatures on the instrument display Refer to Section 6 5 Slot warmup tim...

Страница 45: ...he instrument and unplug the power cable during maintenance procedures Dirty thermal microplate holder contacts Ensure the power contacts on the back of the thermal microplate holder are clean and fre...

Страница 46: ...butor will take care of sending a complaint form that is the Warranty Claim Technical Sheet to the Thermo Fisher Scientific technical service department The Warranty Claim Technical Sheet contains a m...

Страница 47: ...y 100 120 Vac 50 60 Hz nominal 200 240 Vac 50 60 Hz nominal Power consumption 230 VA max Heat dissipation 785 BTU max Fuses 2 x 3 0 A 250 V UL198G Time Delay 5 x 20 mm IEC 127 3 III Operating conditio...

Страница 48: ...es of wells A1 A12 H1 H12 and E7 after 1 hour at 65 C with an ambient temperature of 24 C with 200 l water well with a plate sealer and without shaking Uniformity 0 6 C across the entire plate calcula...

Страница 49: ...ote 1 Pollution degree 2 according to IEC 60664 1 see Note 2 Note 1 The installation category overvoltage category defines the level of transient overvoltage which the instrument is designed to withst...

Страница 50: ...1010 2 010 1994 A1 1996 including CENELEC Common Modifications US and CA National differences EN 61010 1 2001 Ed 2 EMC performance EN 50081 1 1992 Generic emission standard Residential commercial and...

Страница 51: ...3 thermal microplate holders HT 220 240 V 5112257 iEMS Incubator Shaker HT with three 3 thermal microplate holders HT 100 120 V 5921210 iEMS thermal microplate holder HT 10 1 List of accessories and r...

Страница 52: ...from Thermo Fisher Scientific or from an authorized Thermo Fisher Scientific distributor The guarantee is not transferable to a third party without prior written approval from Thermo Fisher Scientific...

Страница 53: ...s warranty limitations 49 Contact information 57 Control LEDs 10 23 39 D Decontamination 36 38 Certificate of 55 instrument surface 37 procedure 36 thermal microplate holder 37 Dimensions 44 Directive...

Страница 54: ...oard 12 cleaning 34 Keyboard keys 12 L LC display 12 M Mains supply cable 17 Mains switch 17 Maintenance 34 Microplate disposal of 36 42 placing of 24 Microplate holder 13 O Operating conditions 15 Op...

Страница 55: ...tocol 43 Setting parameters 20 Setup 18 Shaking 32 pausing 33 speed 26 39 41 time 21 Site of operation 15 Slot status 23 Slot temperature 41 Slot warmup time 41 Specifications 44 general 44 incubator...

Страница 56: ...ell formats for example 6 12 24 48 96 384 864 etc wells for ease of use in performing multiple tests through techniques such as EIA or ELISA Orbital shaking The shaking of a microplate in a circular m...

Страница 57: ...h mm ss Interval time hh mm ss Shaking speed as shown below Value 0 1 2 3 4 5 Shake speed rpm 0 400 650 900 1150 1400 5 Press temp followed by the slot number to start the preheating of the thermal mi...

Страница 58: ...g Before returning any instrument s or item s ensure they are fully decontaminated Confirm A or B status Name Address Tel Fax Name Serial no A I confirm that the returned items have not been contamina...

Страница 59: ...s Tel Tel Fax Date of delivery Internet home page Date of purchase Your research area Dr Mr Mrs Ms Job title Position Surname block capitals First name block capitals Internet e mail address Excellent...

Страница 60: ...Cat no 1506400A 57 Appendix D Addresses For the latest information on products and services visit our websites at http www thermo com Manufactured by Distributed by Thermo Fisher Scientific Oy P O Bo...

Страница 61: ...Thermo Scientific iEMS Incubator Shaker HT User Manual Rev 2 4 Cat no 1506400A 58...

Страница 62: ...Thermo Scientific iEMS Incubator Shaker HT User Manual Rev 2 4 Cat no 1506400A 59...

Страница 63: ...Thermo Scientific iEMS Incubator Shaker HT User Manual Rev 2 4 Cat no 1506400A 60...

Страница 64: ......

Страница 65: ...Thermo Fisher Scientific Oy Microplate Instrumentation Ratastie 2 P O Box 100 FI 01621 Vantaa Finland www thermo com 1506400A...

Страница 66: ...service in house repair center WE BUY USED EQUIPMENT Sell your excess underutilized and idle used equipment We also offer credit for buy backs and trade ins www artisantg com WeBuyEquipment REMOTE IN...

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