EuroFlow EF and EF/B
Biological Safety Cabinet
UM-EFAB-EN10.doc
76
Appendix V: Measuring method air velocities
BIOLOGICAL DANGER
BE CAREFUL!!
Before starting service with the cabinet a written declaration “that the
cabinet is decontaminated” must be present. The responsible person
(laboratory supervisor) will decide if the cabinet has to be decontaminated so
that when servicing the installation and the HEPA-filters there is no danger
for the service engineer, labour personnel, the laboratory and the
surroundings of the cabinet. If necessary disinfect the installation. For more
information
Appendix II: Disinfection on page 67
.
CYTOTOXIC DANGER
When cytotoxics are used, see
§2.5 Cytotoxic danger (EF/B only) on page
10.
Only the responsible safety officer (biological safety officer or the responsible
examiner) can decide if this procedure is valid or not. Additional standard
operation procedures can be necessary.
The measurement of air velocities in the cabinet is performed according to the EN 12469:2000.
Air velocities downflow
•
Switch on the cabinet;
•
Measure the airspeeds according to the pattern of
Figure 43
with an anemometer at a height
of 100 mm above the front opening (See
Figure 42
). This measurement must be done for at
least one minute on each position.
Air velocities inflow*
1
•
Switch on the cabinet;
•
Measure with an anemometer the average air velocity in the inflow, according to the pattern
of Figure 41 and with probe position as shown in
Figure 40
.
•
Multiply these numbers with the surface of the exhaust opening: the result is the air volume
through the safety cabinet;
•
Divide the air velocity through the surface of the front opening: the result is the average
value of the inflow air speed.
