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EuroFlow EF and EF/B

Biological Safety Cabinet

UM-EFAB-EN10.doc

Chloormethine
(Mechlorethamine)

51-75-2

Dactinomycine 50-76-0
Estramustine 2998-57-4

0,1 N Natronloog (Sodium

Hydroxide) CAS-no. 1310-73-2.

Example 3:
Cytotoxic

CAS-no. Recommended

cleaner

Mitomycine

C= 50-07-7

0,1 N HCL (Hydrochloric Acid)
CAS-no. 7647-01-0.

Содержание Clean Air EuroFlow Series

Страница 1: ...inet Class II Types EF 4 EF 5 EF 6 EF B 4 EF B 6 Offers protection for personnel environment and product Before using the safety cabinet please read this manual carefully The required instructions hav...

Страница 2: ...possibility to incorporate weighing systems microscopes etc or even build completely custom made cabinets Furthermore type EF and EF B are service friendly installations The safety cabinet is equippe...

Страница 3: ...______________________15 4 5 Top hood ____________________________________________________________15 4 6 Worktop _____________________________________________________________15 4 7 Aerofoil___________...

Страница 4: ...her alarms _________________________________________________________49 7 Cleaning_________________________________________________ 51 7 1 Cleaning the installation ____________________________________...

Страница 5: ...microprocessor _______________________________________________84 Appendix X User code__________________________________________ 87 Activating the user code ___________________________________________...

Страница 6: ...ulate Air the working area of the cabinet is free of particles This airflow is laminar the airflow has got no turbulence and has got a constant air speed Through the work opening C air is drawn into t...

Страница 7: ...ow has got no turbulence and has got a constant air speed Through the work opening C air is drawn into the workspace inflow towards the front slots of the worktop This protects the personnel against p...

Страница 8: ...rized persons Operating the installation The cabinet shall only be used by personnel which is familiar with the content regulations and warnings which are mentioned in this operating manual is familia...

Страница 9: ...disrupt the airflow In addition all centrifuges themselves create an airflow which may fling particles out of the casing 7 When homogenizers are used in the installation all reservoirs have to be sec...

Страница 10: ...o be evaluated and documented industrial safety act hazardous substances regulations Based on these findings the employees must be instructed 2 6 Explanation user safety symbols GENERAL DANGER You can...

Страница 11: ...laining drawing Mount the support yoke Pos 1 to the reinforcement plate Pos 3 Mount optional feet support Pos 2 Item no 6 is used to position the cabinet to the support frame see 3 1 Placing the cabin...

Страница 12: ...transported on a strong solid pallet After every transport the cabinet must be tested and calibrated GENERAL DANGER Be careful High center of gravity crossover point 3 3 Dismantling Disposal For dism...

Страница 13: ...EuroFlow EF and EF B Biological Safety Cabinet UM EFAB EN10 doc 13 4 Product description Figure 3 Sectional view EF Figure 4 3D view EF Figure 5 Sectional view EF B Figure 6 3D view EF B...

Страница 14: ...pe plate Figure 3 Figure 4 Figure 5 and Figure 6 Pos 1 mentions instructions that are important for the connection of the biological safety cabinet See Appendix VIII Stickers on page 81 4 2 Short user...

Страница 15: ...ard including engine control Additional option print If you wish to check one of these components you need to hinge the top hood To check the interface board the safety cover has to be taken away auth...

Страница 16: ...lass H14 in accordance with EN1822 After assembling each filter will be tested separately according to EN 12469 2000 Annex D Through the topside of the cabinet the exhaust filter can be reached 4 13 S...

Страница 17: ...2 General specifications on page 61 for details 4 18 Electronics The electronics Figure 3 Figure 4 Figure 5 and Figure 6 Pos 18 consist of Operational print positioned in the top hood behind the opera...

Страница 18: ...oltage drops or filter pollution With an installation with a double exhaust HEPA filter an additional fan is built in The fan regulations are mounted behind the light hood 4 22 Optional Fittings Figur...

Страница 19: ...h set The Bunsen burner can be plugged into the outlet with a short cable inside the work space The plug of the foot pedal can be inserted into an outlet The Bunsen burner can only operate when the ca...

Страница 20: ...The UV light is meant for disinfection of the working surface of the safety cabinet When the UV light switches on the FL lighting switches off The UV light can only be switched on when the window is...

Страница 21: ...tions on page 61 for electrical details The constructions of these may vary per country When the Biological Safety Cabinet has been switched off this function has to be activated each time via the swi...

Страница 22: ...by putting the plug into a grounded socket that is easy accessible 5 2 Top hood sliding window The hinge slide window can be in several positions see Figure 8 Window positions The used example of the...

Страница 23: ...e cleaned but also for placing large objects in the working area The window can only be hinged when the window is slit downwards on the aerofoil and when the locking pins are locked see Figure 12 and...

Страница 24: ...window Figure 10 Hinge instructions Figure 11 Locking pins Figure 12 Hinge position Figure 13 Slide position Figure 14 Swivel blocks Figure 15 Hinge position Figure 16 Slide position After hinge actio...

Страница 25: ...est line gives the description of the function key underneath I II III IV Cabinet switched off not safe Monday 11 06 33 MENU Socket Off Fixed keys Lighting ON OFF Work mode fan ON OFF Power save mode...

Страница 26: ...window Put the window in the working position see marks on the installation 3 The fan starts Cabinet is in start up mode The alarm indicator light is red There is an acoustic alarm Push button To ack...

Страница 27: ...hapter 9 2 on page 61 Measuring these most critical measured values directly under the window ensures that the overall average inflow velocity is according to the guidelines of the EN12469 2000 Annex...

Страница 28: ...Push button When the cabinet is locked enter the user code To acknowledge this alarm push button Cabinet in power save mode not safe Downflow to low Inflow MENU Socket Off 2 Slide down the window onto...

Страница 29: ...be switched off Enter the user code and push button Cabinet switched off Not Safe Monday 11 06 33 MENU Socket Off 5 8 Control UV optional The UV function is only visible if this option is installed an...

Страница 30: ...supply optional The gas function is only visible if this option is installed and the cabinet is in work mode with no air alarms In all other cases the gas valve will automatically be switched off and...

Страница 31: ...or maximum voltage and current fuses Switching socket on 1 Cabinet in work mode See 5 4 Starting up the cabinet on page 26 Cabinet in work mode Safe Monday 11 06 33 MENU Socket Off 2 The display shows...

Страница 32: ...mode See 5 4 Starting up the cabinet on page 26 Push button Menu Cabinet in work mode Safe Monday 11 06 33 MENU Socket Off 2 Push button A A Hour counter information B Status cabinet C Settings A B C...

Страница 33: ...or starting menu Set up 7 days clock UV Cabinet in work mode See 5 4 Starting up the cabinet on page 26 Push button Menu Cabinet in work mode Safe Monday 11 06 33 MENU Socket Off 1 Set up for 7 days c...

Страница 34: ...e en ending time The value can be changed by pushing button or Acknowledge the setting with button Push 2x button Esc for starting menu See also the Example on page 34 Disable 2c By pushing on Disable...

Страница 35: ...sc 2 Set up the UV 7 days clock Push button Clock for setting up the UV 7 days clock Push button Change for changing the start and end time The value can be changed by pushing button or Saturday UV on...

Страница 36: ...utton Change Tuesday has to be changed into Wednesday UV starting time at midnight 00 00 and UV off at 06 00 The display shows Acknowledge the setting with button Push 2x button Esc for starting menu...

Страница 37: ...clock 1 Cabinet in work mode See 5 4 Starting up the cabinet on page 26 Set up display for socket 7 days clock shows Push button Menu Push button A Push 2x button Acknowledge the setting with button...

Страница 38: ...binet It is however not possible to switch off the cabinet itself automatically 1 Cabinet in work mode See 5 4 Starting up the cabinet on page 26 Push 5x button 1x Menu 1 x A and 3x Acknowledge the se...

Страница 39: ...sc 6 Acknowledge the setting Acknowledge the setting with button Push 2x button Esc for starting menu For more days hours minutes walk again through the menu and confirm each time Set up actual time 1...

Страница 40: ...nform service personnel In information status more details are given about Actual velocities alarm setting and fan settings Information about the installed options Sockets UV Information about Options...

Страница 41: ...chapter 9 2 on page 61 Measuring these most critical measured values directly under the window ensures that the overall average inflow velocity is according to the guidelines of the EN12469 2000 Anne...

Страница 42: ...up from Step 2 Installed options display shows Push button For explanation codes see Appendix IX Information installed options on page 84 Push button Esc for starting menu Installed Options 1 Socket p...

Страница 43: ...e contact Clean Air B V Also check the site of Clean Air B V www cleanair eu products downloads for more detailed information about disinfection Depending on the code of the installation the safety ca...

Страница 44: ...self is no disinfection Activating the user code See Appendix X User code on page 87 Factory adjustments 1 Cabinet in work mode See 5 4 Starting up the cabinet on page 26 Cabinet in work mode Safe Mon...

Страница 45: ...net on page 26 Cabinet in work mode Safe Monday 11 06 33 MENU Socket Off 2 Testing of alarms display shows Push button and C Push 3x button Push button Enter service code Testing of alarms 0000 Enter...

Страница 46: ...The cabinet will be switched on automatically 1 Cabinet in work mode See 5 4 Starting up the cabinet on page 26 Cabinet in work mode Safe Monday 11 06 33 MENU Socket Off 2 Change the language Push bu...

Страница 47: ...sound pressure level of at least 10 dB A more than the sound pressure of the working cabinet See Appendix XI Required specifications on page 89 In one band of the Terz band analysis mentioned in the...

Страница 48: ...f the cabinet which helps to find possible causes see 5 13 Information status cabinet on page 40 Downflow too low Downflow too low Figure 20 Product protecting downflow is too low Product protection b...

Страница 49: ...ikely the fuse is broken Replace it with a new one If the alarm is still there contact your service organization To reset this alarm you must disconnect the power plug from the main voltage Communicat...

Страница 50: ...n the alarm will not mute immediately The cabinet needs time to get the correct airflows In case the alarm is still present Stop working close the window and push button The cabinet goes into the savi...

Страница 51: ...he worktop see 4 6 Worktop on page 15 the space under the worktop and drip trays regularly A 1 solution of Natriumdodecylsulfat SDS solution CAS no 151 21 3 in demineralised water could be applied for...

Страница 52: ...and below side of the aerofoil see 7 1 Cleaning the installation on page 51 Clean all visible parts which are normally covered by the aerofoil see 7 1 Cleaning the installation on page 51 Place the a...

Страница 53: ...fect the window with liquid Put on gloves Switch off the installation see 5 7 Turn off on page 28 Hinge the window open see 5 2 Hinge the window on page 22 Clean and if necessary disinfect the window...

Страница 54: ...Cabinets contaminated with cytotoxics are normally cleaned with a 1 solution of Natriumdodecylsulfat SDS solution CAS no 151 21 3 in demineralised water as described in 7 1 Cleaning the installation...

Страница 55: ...10 doc 55 Chloormethine Mechlorethamine 51 75 2 Dactinomycine 50 76 0 Estramustine 2998 57 4 0 1 N Natronloog Sodium Hydroxide CAS no 1310 73 2 Example 3 Cytotoxic CAS no Recommended cleaner Mitomycin...

Страница 56: ...abinet Contact your service organization for advice Guideline for testing and maintenance given by Clean Air Techniek B V is every 12 months at least Validate the system on safety Check for correct fu...

Страница 57: ...rements are printed on the type plate see 4 1 Type plate on page 14 and Appendix VIII Stickers on page 81 Figure 28 Supplies needed Procedure Switch on the installation Make sure that the window is in...

Страница 58: ...e plastic bag inside the front aperture with your left hand Figure 31 Positioning the left hand in the plastic bag Figure 32 Positioning the plastic bag on the aerofoil Positioning the plastic bag on...

Страница 59: ...d the worktop back in its position Remove the gloves and put them in the appropriate container 8 3 Replacing the Fluorescent light FL light When an FL light see 4 20 FL lighting on page 18 needs to be...

Страница 60: ...e HEPA filters on page 74 Alarm tests see Appendix VII Alarm test procedure on page 79 Check visual if the air direction of the inflow is in direction of the front aperture and laminar over the whole...

Страница 61: ...4 EF B 6 Front aperture work mode hxl mm 165x1190 165x1495 165x1800 165x1190 165x1800 Front aperture maximum height mm 350 350 350 350 350 Exteriors dimensions without support frame without exhaust h...

Страница 62: ...acc EN 1822 First HEPA filter HEPA V pleats Type PB H14 H14 acc EN 1822 8 pieces HEPA V pleats Type PB H14 H14 acc EN 1822 12 pieces Performance EF 4 EF 5 EF 6 EF B 4 EF B 6 Sound level according to...

Страница 63: ...5 230 3 15 Power sockets Pmax W 725 725 725 725 725 Power consumption in standby mode with light switched off excl maximum power socket P J s W I A 303 1 3 513 2 2 568 2 5 533 2 3 766 3 3 Power consum...

Страница 64: ...1 6 1 6 F9 PCB A T 1 6 1 6 1 6 1 6 1 6 Fuses option board EF 4 EF 5 EF 6 EF B 4 EF B 6 F1 Optional fan A T Max 8 Max 8 Max 8 Max 8 Max 8 F2 Optional A T Max 8 Max 8 Max 8 Max 8 Max 8 F3 Charge curren...

Страница 65: ...ot working Window not down Window completely downwards Gas equipment is not installed x Gas button is not visible on display Option gas is not installed UV option has to be programmed please contact y...

Страница 66: ...ological Safety Cabinet UM EFAB EN10 doc 66 Appendix I Support frames EF EF B For every type of cabinet from the EF series and the EF B series a specific support frame is available Figure 35 Example o...

Страница 67: ...control print is only available in version number 2 4 270307 or higher The disinfection software itself is no disinfection The Biological Safety Cabinet can work according to automatic disinfection so...

Страница 68: ...officer or the responsible examiner can decide if this procedure is valid or not Additional standard operation procedures can be necessary The valid procedures for the disposal of possible contaminate...

Страница 69: ...t within specifications and or when it is not possible to adjust the air velocity according the specifications For the first V shaped HEPA filters only relevant to the EF B class II cabinets the follo...

Страница 70: ...sal procedure Removing the old downflow HEPA filter Decontaminate the cabinet Switch off the installation and plug out the power plug Put on the gloves and keep an extra pair ready for use Put on your...

Страница 71: ...Appendix IV Efficiency test of the HEPA filters on page 74 Replacement of the exhaust HEPA filter For replacement of the exhaust HEPA filter the following procedure must be followed Supplies needed Pl...

Страница 72: ...HEPA filter the following procedure must be followed Supplies needed Plastic bag with closure minimum measurement depends on the volume of the HEPA filter Gloves 4 pair for EF B 4 6 pair for EF B 6 S...

Страница 73: ...inner side of the plastic bag Press the air out of the bag with its opening still in the working space Seal the plastic bag airtight Remove the plastic bag and place it in an appropriate container Rem...

Страница 74: ...ements Efficiency test by means of a particle counter The downflow exhaust and first filter must have an efficiency of 99 995 A locally measured value can be a factor 10 higher 0 05 Efficiency test wi...

Страница 75: ...point upstream concentration for the downflow filter as well as the exhaust filter is positioned under the work area Figure 37 Pos B and Figure 38 The downflow filter and the exhaust filter can be sc...

Страница 76: ...biological safety officer or the responsible examiner can decide if this procedure is valid or not Additional standard operation procedures can be necessary The measurement of air velocities in the c...

Страница 77: ...The minimal required value of the average inflow velocity measured at the top of the front aperture is given in chapter 9 2 on page 61 Measuring these most critical measured values directly under the...

Страница 78: ...oFlow EF EF B Biological Safety Cabinet Clean Air Techniek B V UM EFAB EN10 doc 78 Appendix VI Lay out boards Interface board Figure 44 Interface board Control board Figure 45 Control board E010001 re...

Страница 79: ...touched the fan returns to its work mode Alarm test procedure 1 Cabinet in work mode See 5 4 Starting up the cabinet on page 26 Push button Menu Push button C Cabinet in work mode Safe Monday 11 06 33...

Страница 80: ...37 m s The fan speed is shown in tenths of percents 0 min and 1000 max 1 The average inflow velocity at the top of the front aperture is measured and can be read in the display The minimal required va...

Страница 81: ...ticker cabinet type This sticker can be found on the outside underneath the cabinet For an example of the sticker see the picture underneath 2 Sticker filter type This sticker can be found behind the...

Страница 82: ...ticker has to positioned in sight of the operator 8 Cytotoxic only EF B This sticker is delivered with the cabinet Depending on the work that is done on the cabinet the customer must decide whether th...

Страница 83: ...s sticker is delivered with the cabinet Depending on the work that is done on the cabinet the customer must decide whether there are any Cytotoxic hazards or not In case of any Cytotoxic hazards the s...

Страница 84: ...the airflow in the exhaust chamber In this case it is recommended to remove the exhaust hood or contact Clean Air Techniek B V for advice For the same reason the exhaust channel may not be obstructed...

Страница 85: ...are factory adjustments and cannot be changed by the user Overview functions of relay K3 B1 B5 Alarm B1 B5 relay switching K3 on output J22 at the IO print B1 the K3 relay is activated if the main fan...

Страница 86: ...th intervals To install alarm limits start from the menu as described in 5 13 Information about the installed options on page 41 The display only shows when the options are installed 1 Starting from s...

Страница 87: ...is a special code to break the unknown or forgotten user code In that case enter code 2882 and the original pre installed code 0001 is valid again 1 Cabinet in work mode See 5 4 Starting up the cabin...

Страница 88: ...Push button Menu and C Push 4x button Push button Enter actual user code Push button Enter the new valid user code Push button User code 0000 Enter code A Enter Esc Input user code New code 0000 Old c...

Страница 89: ...12469 Annex A 6 Type test Yes Glass and front windows Laminated safety glass or UV resistant safety plastics EN 12469 Annex A 7 Type test Yes Glass and front windows Conform to EN 12100 1 2003 and 121...

Страница 90: ...and front windows Laminated safety glass or UV resistant safety plastics DIN 12980 Chapter 6 Type test Yes Electrical safety Conform to EN 61010 1 DIN 12980 Chapter 11 Type test Yes Ergonomics Mainte...

Страница 91: ...9S2R3L 15P6 610X1524X69 mm H14 acc EN 1822 Exhaust HEPA filter EF 5 1000606 AAF Astrocell A99C9S2R3 6P6 610X610X69 mm H14 acc EN 1822 Pre filter EF 5 1050001 G3 EN 779 Torsion spring EF 5 2700675 Cold...

Страница 92: ...ll 6400010 Acrotronics F BK D 3940 ZG 10A PCB material All Duraver FR 4 according to UL94 Socket All Misc e g Mennekes Schuko Optional Gas vacuum tap All 2800013 2800048 Broen 1770 Gas valve All 28200...

Страница 93: ...EuroFlow EF and EF B Biological Safety Cabinet UM EFAB EN10 doc 93 Appendix XIII Short user manuals Figure 49 Short user manual EF series Figure 50 Short manual EF B series...

Страница 94: ...EuroFlow EF and EF B Biological Safety Cabinet UM EFAB EN10 doc 94 Appendix XIV TUV Low Pressure Mercury Lamps...

Страница 95: ..._______________________ is in compliance with the following directives Machine Directive 2006 42 EC Low Voltage Directive 2006 95 EC Electromagnetic Compatibility Directive 2004 108 EC And declares f...

Страница 96: ...EuroFlow EF and EF B Biological Safety Cabinet UM EFAB EN10 doc 96 Appendix XVI Memo...

Страница 97: ...xhaust HEPA filter 6 7 F Factory adjustments 44 Fan 6 18 Feet Support 16 Fixed keys 25 FL light 59 FL lighting 18 Front panel 16 Function keys 25 G Gas connection 18 Gas supply off 30 Gas supply on 30...

Страница 98: ...et up socket 7 days clock 37 Short user manual 14 93 Signals 25 Slide window 23 Sliding window 22 Socket off 31 Socket on 31 Sockets 21 Software Version 60 Standby mode 28 Starting up the cabinet 26 S...

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