Teledyne CombiFlash EZ Prep, Руководство пользователя

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CombiFlash® Rf
+
User Manual
5-2
To prepare an empty solid sample cartridge:
1. Ensure the empty cartridge has a bottom frit.
CAUTION
Missing frits may cause equipment damage, UV detection
problems, or increased maintenance. Frits prevent solids from
entering the fluid path.
2. Prepare the media and sample:
a. Dissolve your sample in a minimal amount of a
suitable, volatile solvent.
b. Place the media into the solvent. If using silica gel, a
particle size of 40–60 µm (240–400 mesh) is
recommended. The amount of silica required is about
four to five times the mass of your sample.
c. Agitate the solvent for a moment to allow the sample
to adsorb to the silica.
d. Remove the solvent with a suitable method, such as
rotary evaporation. Alternatively, you can dry the
prepared cartridge after step 5 by drawing the
solvent out using a vacuum or using the Rf
+
system’s
air purge.
3. Load the media and sample mixture into the cartridge. Tap
the cartridge on the bench top to settle the mixture.
4. Place a frit on the top of the cartridge. Force the frit down
against the mixture using the plastic plunger.
5. Wipe any residual powder inside the neck of the cartridge.
Note
On Rf
+
systems, including those equipped with 4x, you can
remove solvent from a prepared cartridge by attaching the
cartridge cap and manually purging (T
OOLS
>M
ANUAL
C
ONTROL
)
the cartridge with air.
Empty solid sample cartridges also allow a variety of
adsorbents, such as diatomaceous earth, boiling chips, cotton
balls, or paper wipes. Even with alternative adsorbents,
always use top and bottom frits to prevent fluid path
problems.