Soft Imaging System ColorView III, Руководство пользователя

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Camera and light microscope
7
Camera and light microscope
Both the camera settings as well as the microscope settings are vital for the
quality of the image to be acquired. Only the most important rules for the mi-
croscope settings are mentioned here; a more thorough explanation to the
topic can be found in the appendix:
1)
The illumination must be parallel.
2)
Use ND-filters to reduce the illumination intensity; do not alter the lamp
voltage.
3)
Establish Köhler illumination conditions.
4)
Select the appropriate total magnification and the objective for the
problem in question.
White Balance
The human eye reacts very sensitively to color variations which are per-
ceived as a color tingeing effect. Therefore, when wanting to acquire quality
images with a digital camera, it is vital that the individual color channel set-
tings are set so that the entire image does not show color tingeing. The ad-
justment of the color channels and the white balance depend on the illumi-
nation conditions. When changing the lamp or altering the lamp voltage, the
white balance should be repeated afterwards accordingly.
When using white balance, the individual color channels are scaled in a way
that the white or neutral gray area of the image displayed on the monitor is
displayed correctly as white or gray. The white balance can be carried out
automatically on each image or manually for individual images.
Mode of operation An area which is, for the most part, uniformly gray or white is selected on the
image. Such an area must contain an equal intensity of the three color com-
ponents : red, green and blue. White balance sets the color channels in such
a way that the requirements for the selected area are satisfied as much as
possible. To be able to carry out an effective white balance, you need the
respective white or gray area.
White balance is carried out on a part of the image (ROI). The program alters
the color settings in such a way that the image area in the ROI appears as
white or gray as possible. After you have once carried out the white balance,
you can reapply it any time to a running live-image acquisition.
Standardized White Bal-
ance
A test image is acquired with the camera using optimal illumination for the
standardized white balance. The individual color channels are now set so
that the acquisition also appears white or gray on the monitor. These chan-
nel settings are saved and used automatically for all additional acquisitions.
Manual White Balance A manual white balance is carried out on the current single image / snap-
shot. To do this, you need an area in the image which is for the most part
gray or white.
Microscope Settings Use the same microscope settings for the white balance that you will use for
the acquisitions later. This especially holds true for the filter and the lamp
voltage settings. The lamp voltage of a 12V halogen lamp should be set to
9V. It should, however, not be set lower than 5V. Use the color neutral ND-
filter, should you have to reduce light intensity.
Related Topics
Appendix I: Microscope Settings 45