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Appendix I: Microscope Settings
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Appendix I: Microscope Settings
Your digital camera can only achieve high image quality if the microscope
has been optimally set. A subsequent correction to the software can never
correct deficiencies in the image resulting from faulty settings made to the
camera and microscope.
The optical system microscope offers numerous setting possibilities. In or-
der to attain best acquisition results, the settings must be made precisely.
In spite of varying models and observation possibilities, the microscope's
image producing components are in accordance with each other.
A microscope basically consists of the optical components: projection objec-
tive and magnifier. The projection objective is the objective with which the
observation is to be carried out. The eyepiece takes on the function of the
magnifying glass. An additionally important component is the illuminator.
This illustration clarifies the
basic assembly of the com-
ponents of a microscope in
transmitted-light mode.
Illumination
Light is a decisive medium for creating images with a microscope. As a result, the light, i.e. illumination,
plays an important role in creating the image in the microscope.
The goal of the illumination settings is the equal distribution of light on the
sample with parallel light rays falling on the object. The illumination must be
bright enough to make all of the image details visible; however over-illumi-
nation leads to images being too bright and low-contrast. In addition, the col-
or of the light has a strong impact on the sample's color rendering.
Lamp Selection The tungsten-halogen lamps and gas discharge lamps are the most com-
monly used lamps in microscopy. The light creating processes of both lamp
types are fundamentally different.