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Online shading correction
9
3)
Click the
Set ROI for White Balance
button.
4)
Pull the ROI's red frame shown in the image document to a position
which is more or less a uniform white or gray.
"
Change the size of the area by keeping the mouse depressed and
moving the mouse. The ROI used for the white balance cannot be
made to any large size possible.
"
The ROI can be difficult to see if the image document has a reddish
shade. In this case, use the
Special > Preferences > View
com-
mand to change the color of the frame. You can select a more ap-
propriate color from the
Primary
list located in the
Mouse cursor
group.
5)
Rightclick to confirm position and size of the ROI.
6)
Click the
White Balance
button to conduct an automatic white balance.
"
The settings for the individual color channels are optimized in the
set area. The result is directly visible in the image buffer's image.
7)
Click the
Color Settings
button.
"
The
Color Settings
dialog box is opened.
8)
Click the
Define...
button.
"
The
Define Default Color Settings
dialog box is opened.
9)
Click the
Current settings
button to adopt the current values for the rest
of the work.
"
When using this setting, the white balance is valid for all images
which are acquired with this input channel during the current ses-
sion.
10)
Click the
OK
button to return to the
Color Settings
dialog box.
11)
Click the
Standard
button to save these values as general standards.
"
The current values for the white balance are now saved and will be
imported when the program is restarted. A manual change of the
color settings changes the white balance.
The program alters the color settings in such a way that the image area in
the ROI appears as white or gray as possible. After you have carried out the
white balance once, you can reapply it any time to a running live-image ac-
quisition.
Online shading correction
Optical systems consisting of camera and microscope generate image inho-
mogeneity, or so called shading, even if care was taken with setting up the
devices.
The left illustration shows an
image where the online
shading correction has not
been activated. In compari-
son to the image on the
right, one can notice the in-
homogoneities in the illumi-
nation.
Содержание ColorView III
Страница 1: ...analySIS Manual ColorView III ...
Страница 6: ...ColorView III 4 ...
Страница 50: ...ColorView III Objective Total Magnification and Useful Magnification 48 ...
Страница 76: ...ColorView III When encountering problems with the camera 74 ...
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