Schambeck SFD GmbH
Refractive Index Detector RI2012
RI2012 V1.0 06-2013.EN / 28.10.2013
64
http://www.schambeck-sfd.com
Use a flash light to check the flow cell for contaminants or damages. Make sure that
no bubbles are inside the flow cell.
If there are contaminants in the flow cell purge it with a suitable solvent. If you are
working with aqueous systems distilled water is convenient. If you use organic
substances try solvents like acetone, tetrahydrofurane or chloroform. After purging
the cell with solvent to clean for a longer time you should purge it with you mobile
phase and check again if the contaminants is removed.
■
NOTE
In the case of dangerous solvents make sure that they are disposed correctly.
■
NOTE
In case of aqueous solvents it is possible that algae grow inside your chromatographic
system. For that reason it is not recommended to store the detector for a longer time with
aqueous solvents inside. For long time storage it is recommended to purge the flow cell with
ethanol followed by air.
If possible we recommend the addition of a small amount of organic solvent (such as
isopropanol or methanol) to your mobile phase to prevent algae growth.
If it is not possible to remove the contaminant from your flow cell it might be necessary to
replace it with a new flow cell. Please contact your local distributor or Schambeck SFD
GmbH directly for assistance.
If the flow cell is cleaned set the slit aperture back in its position and fix it.
Put the lid on the inner housing and tighten the screws.
Place the heat insulation back on top of the inner housing.
Close the outer housing and tighten the screws.
Perform a measurement to check if the problem is solved now. If you still have problems
using your RI2012 refractive index detector please contact you local distributor or
Schambeck SFD GmbH directly for assistance.
