Schambeck SFD GmbH
Refractive Index Detector RI2012
RI2012 V1.0 06-2013.EN / 28.10.2013
17
http://www.schambeck-sfd.com
11 I
NSTALLATION OF THE
RI2012
11.1 C
ONNECTING THE DETECTOR TO THE CHROMATOGRAPHY SYSTEM
Connect the INLET on the front of the detector to the capillary which comes from the column
of you chromatography system. To attach the column to the de
tector use a 1/16” stainless
steel capillary with 0.25 mm inner diameter.
Connect the 1/16” stainless steel capillary with 1 mm internal diameter to the PTFE tube
and the OUTLET port of the instrument. Use the screw connections to fix the capillaries to
the INLET and OUTLET port.
Place the free end of the PTFE tube in a bottle for solvent waste. The droplets leaving the
tube should run along the bottles wall and not fall down. The waste bottle and the refractive
index detector should be positioned at the same high level.
If you need a solvent mixture to perform your analytical separation it is recommended to mix
the different components manually before using the solvent. The use of a gradient pump to
generate a solvent mixture is not possible as the mixture generated by the gradient pump is
not consistant. Small changes in the solvent compositions will result in unstable detector
signals.
It is also recommended to degass the solvents before use with a refractive index detector as
this will result in a more stable detector signal.
■
NOTE
Before dispatch of the instrument the flow cell was purged first with ethanol followed by air.
However there are still some rests of ethanol in the flow cell. For transportation or long time
storage of the instrument the procedure of purging with ethanol and air is recommended.
■
NOTE
The flow cell is built of specialized optical glass which is sensitive to pressure. The
maximum pressure for the flow cell is
6 bar
(
0.6 MPa
). If you want to use several detectors
is series make sure that the refractive detector is the last one in the row to prevent damages
of the flow cell due to high pressure.
