Sakura Tissue-Tek Xpress x120, Руководство по эксплуатации, Страница: 79 / 82

Revised 10/02/2012 A.5
Microscopic Evaluation
Moderate swelling of red blood cells may be noted.
Artifact - A retraction artifact that may be observed
between epithelial cells and the underlying connective
tissue when tissue is placed directly into Molecular
Fixative or if the tissue was insufficiently fixed. The artifact
may appear as a halo that surrounds the tissue cell(s).
The artifact may also be noted on tissues that have been
processed using Formalin fixation. This artifact may
appear more prominent on Molecular-fixed tissue.
Reprocessing of Tissue
Tissue-Tek Xpress x120-processed tissue may be
reprocessed, if necessary, without adverse affects on the
tissue. Tissue that has been trimmed too thick for proper
processing can be reprocessed using the following
reprocessing procedure
.
Procedure:
1. Place tissue cassette in a 60°C oven or in the Tissue
Embedding Center and allow the block to melt down.
2. Remove tissue and place in a cassette identified with
the proper accession number. It is important to note
the thickness of the residual tissue.
3. Place labeled cassette in solvent (xylene or
xylene substitute) for 30 minutes (two changes).
4. Place cassette with tissue in a beaker containing
absolute alcohol for 30 minutes (two changes).
5. Remove cassettes from the alcohol.
6. Retrim tissue to the correct thickness, as necessary.
7. Place tissue in processing basket, then place the
basket in Pre-Processing container filled with Pre-
Processing Solution for 15 minutes.
8. Place processing basket in the Loading Station, with
the preprocessing step enabled and set for 30
minutes.
9. Depending on the thickness of the residual tissue,
select the appropriate immersion program (2mm or
3mm).
10. Enter number of cassettes loaded, as required.
11. At completion of the processing program, remove and
embed tissue as usual.
Tissue-Tek Xpress x120 Reagent
Replacement Schedule
It is recommended that Tissue-Tek Xpress x120 rea-
gents be replaced after processing up to approximately
1,500 cassettes, or up to every 3 days, whichever occurs
first. Reagent integrity depends on the type of tissues
processed. Reagents cannot be topped off or rotated. All
reagents must be replaced at the same time to ensure
proper processing of tissue samples.
NOTE: The user may want to change the reagent set
more frequently depending on the tissue type or shifts of
operation.
Pre-processing solution in the Loading Station should
be changed every 3 hours.
Signs of reagent over-use are:
•
The odor of vinegar is noted when opening the
Paraffin Ovens lids.
•
Paraffin feels slippery or oily when the tissue is
embedded.
•
White areas are noted on the majority of the uncut
paraffin blocks.
•
Tissue “disintegrates” when ribbon is floated on the
waterbath.