Sakura Tissue-Tek Xpress x120, Руководство по эксплуатации, Страница: 76 / 82

APPLICATIONS MANUAL
A.2 Revised 10/02/2012
minutes. Fatty tissue, lymphoreticular tissue and bloody
tissue may require additional immersion time (30 – 60
minutes) in the Pre-Processing Solution prior to perform-
ing the final trimming of the specimen. Following the
required immersion time in the Pre-Processing Solution,
the cassettes are then removed from the solution, opened,
and the tissue then trimmed to the final 2 mm in thickness.
The tissue is then placed back in the cassette, and is
immersed in 10% NBF for 1 hour.
CAUTION: Tissue Specimens are to be held in 10%
NBF until they are ready to start the preprocessing
step.
NOTE: It is recommended that tissue specimens are
immersed in Pre-Processing Solution for a minimum of 45
minutes or in Pre-Processing Fixative for a minimum of 30
minutes prior to being processed by the Tissue-Tek
Xpress x120.
Tissue Preparation for the 3 mm
(30min/station) Extended Immersion
Program
The 3 mm Extended immersion program is used for
processing of tissue specimens of up to 3 mm in thick-
ness. The total processing time, including robotic transfer
of the basket between stations, is approximately 127
minutes. The length and width of the tissue should allow
for space between the tissue and cassette walls.
The tissue is trimmed to 3 mm maximum in thickness,
placed in cassettes, and is immersed in 10% NBF for 3
hours.
NOTE: All tissue must be immersed in Pre-Processing
Solution for a minimum of 15 minutes prior to being
processed by the Tissue-Tek Xpress x120.
CAUTION: Tissue specimens are to be held in 10%
NBF until they are ready to start the preprocessing
step.
Processing Tissue Specimens
CAUTION: The Loading Station retort should be filled
with Preprocessing Solution to accelerate the
dehydration process.
Additional processing baskets (with tissue samples)
can be loaded into the Loading Station when the Loading
Indicator lights steady “ON” and the Loading Station
Access Permitted Icon displays on the Process Monitor
screen.
At the completion of the processing program, the bas-
ket containing the processed cassettes may be removed
immediately. The processed cassettes are then transport-
ed to the embedding center. Each processing basket must
be thoroughly cleaned before reuse (see “
Cleaning of
Processing Baskets” on page 7.1 for details).
Use of Tissue-Tek Molecular
Fixative
Use of Molecular Fixative is mandatory for tissue spec-
imens that require certain studies, such as DNA, RNA,
and protein. It is recommended that specimens for RNA
studies be placed into Molecular Fixative
immediately
upon excision from the patient. The use of Molecular
Fixative
:
•
Stops any further degradation of RNA that normally
occurs
•
Does not cause cross linking with proteins
•
Preserves histomorphology
•
Protects high quality DNA, RNA, and proteins at
ambient temperature
•
Can be used to transport the specimen to the laboratory
•
Can be used for tissue specimen storage
NOTE: Pre-Processing Fixative is not as compatible with
all Molecular Pathology techniques (it is however compat-
ible with IHC techniques, the same as Pre-Processing
Solution).