Qiagen PyroMark Q96 ID, Руководство пользователя

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Contents
Contents-2 PyroMark Q96 ID User Manual 01/2016
3.8.2 Rear panel 3-7
3.9 Computer and software 3-8
4 Installation Procedures 4-1
4.1 Instrument delivery and installation 4-1
4.2 Requirements 4-1
4.3 Moving the PyroMark Q96 ID 4-3
4.4 Reinstalling the PyroMark Q96 ID: Stand alone 4-4
4.5 Reinstalling the PyroMark Q96 ID: Network installation 4-4
4.6 Connecting mains power 4-5
4.7 Setting up network properties 4-5
4.7.1 Used IP addresses for instrument and computer 4-5
5 Operating Procedures 5-1
5.1 Required items 5-1
5.2 Starting the system 5-1
5.2.1 Starting the PyroMark Q96 ID 5-1
5.2.2 Starting the PyroMark Q96 Software 5-2
5.3 Preparing a run 5-2
5.3.1 Setting up an assay 5-2
5.3.2 Setting up a run 5-3
5.3.3 Managing instrument parameters 5-4
5.4 Sample preparation 5-6
5.4.1 Assembly of the PyroMark Q96 Vacuum Workstation 5-7
5.4.2 DNA amplification 5-7
5.4.3 Immobilization of the PCR product to beads 5-8
5.4.4 Strand separation 5-9
5.4.5 Annealing of sequencing primer to samples 5-14
5.4.6 Reagent preparation 5-14
5.5 Run 5-15
5.5.1 Loading the PyroMark Q96 Plate Low 5-15