42
Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516
TM411 · Revised 4/15
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5.B. Starting a Protocol (continued)
7. Set up the deck tray and instrument as indicated on the Extraction Checklist screen. The steps necessary
for setting up the instrument are:
• Sample preprocessing is complete. Sample preprocessing, if required, is described in the Technical
Manual for the specific Maxwell
®
RSC reagent kit.
• Place cartridges at the selected positions in the deck tray (Figure 42). Press down firmly to snap the
cartridges in place at both ends. There should be an audible click.
Figure 42. Placing the cartridges in the Deck Tray and pressing firmly to snap them in
place.
• Completely remove seals from all cartridges.
• Place elution tubes at the selected positions in the deck tray.
• Add the appropriate volume of elution buffer to each elution tube (see the Maxwell
®
RSC reagent
kit Technical Manual for the correct volume to use). Leave the elution tube lids open.
• Add preprocessed sample to well #1 (nearest the clip end) of the cartridge.
• Ensure no plungers from the previous run(s) are present on the plunger bar inside the instrument.
If there are plungers present, go to Section 5.C for instruction for plunger removal.
• Place a plunger in the last well (closest to the elution tube) of each cartridge.
• Place the deck tray in the instrument as shown in Figure 43. Ensure that the deck tray is completely
seated inside the instrument.
11240T
A
11241T
A
