![background image](http://html1.mh-extra.com/html/pp-systems/ciras-3/ciras-3_operating-manual_1592668092.webp)
CIRAS-3 Operation Manual V. 1.09
92
phiPSII-SP (
ϕPSII-Single-Pulse)
. If selected, a single, saturating pulse will be
applied to the light-adapted leaf sample. The single pulse duration and
intensity can be set in the lower part of the screen under “Saturating Light”.
phiPSII-MP (
ϕPSII-Multi-Pulse)
. If selected, multiple, saturating pulses will be
applied to the light-adapted leaf sample. The Multi-Pulse duration and
intensity settings (up to 5 steps) can be set in the lower part of the screen
under “Saturating Light”.
ϕPSII.
If this is selected, a saturating pulse will be applied to the light-adapted
leaf sample after a photosynthesis measurement has been recorded.
phiPSII-FoP (
ϕPSII-Fo’).
If this is selected, a saturating pulse will be applied
to the light-adapted leaf sample followed by illumination using the far-red light
for measurement of Fo’ after a photosynthesis measurement has been
recorded.
Fs
. If this is selected, the steady state fluorescence will be recorded after a
photosynthesis measurement is recorded.
Enter the estimated
Total Manual or Timed Repeated Records
for the
measurement session you will conduct. For example, if you will make
measurements in Manual recording mode, and you know that you will apply 5
different light levels for which you would like to record chlorophyll fluorescence
once with far-red light application immediately following each saturating pulse,
select
ϕPSII-Fo’ after every 1 records,
and
Total Manual
or
Timed
Repeated Records
=5. If using a Response Curve to measure fluorescence,
Repeated Fluorescence will be used in conjunction with Records per Level in
the response curve.
To make only a single Initial measurement (for example, to dark-adapt and
perform Fv/Fm on multiple leaf samples) set
Total Manual or Timed
Repeated Records
=0.
Concluding
Fluorescence
Measurement
This defines the final measurement to be recorded after the last
photosynthesis measurement.
None
. If this is selected, there will be no concluding chlorophyll fluorescence
measurement and the sequence will terminate after the last photosynthesis
measurement.