OxyFlo Pro User Manual
Revision 1.1 | May 2015
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To use the same probe in multiple channels, the calibration procedure must
be repeated in each individual channel.
Every probe features a probe ID number (between 1 and 26) that the
monitor ‘reads’ each time the probe is connected. This is confirmed on the
display toolbar and on the probe status dialogue.
Probe identification numbers are assigned such that no two probes (issued
to the same user) feature the same ID.
The exception to this rule are combined blood flow and oxygen/temperature
sensors (requiring the counterpart OxyLite Pro monitor), which feature a
fixed ID number (25).
There is no recommended re-calibration interval for probes; generally
speaking probe calibration coefficients should remain valid for a number of
months or even years. However, where an experiment requires the
simultaneous use of multiple probes it may be sensible to ensure all are
freshly calibrated prior to use.
Calibration Procedure
To perform a new probe calibration, you will require a Calibration Kit
(product code CAL KIT) which consists of a motility standard and a
positioning clamp. The motility standard is a solution of latex spheres at a
precisely defined concentration. The positioning clamp is used to maintain
the probe in the solution.
IMPORTANT: In order for the calibration to succeed, it is crucial that the
probe and calibration solution are not subjected to vibration or movement of
any kind during the calibration procedure.
IMPORTANT: Do not use the motility standard in ambient temperatures
below 15ºC or above 25ºC.
Proceed as follows to calibrate a probe:
1. Gently swirl the bottle to disperse the contents before use.
2. Open the bottle and allow the contents to settle for one minute
before proceeding.
3. Carefully position the probe into the solution. This is best achieved
by grasping the probe cable within the jaws of the clamp and
carefully lowering the active area of the probe into the centre of the
solution as shown: