1. Adjust the focus of JuLI™ FL (Refer to page 14-15).
2. After adjusting focus, press the
Auto focus button
to get more
accurate focus without individual variations.
· The
Auto focus
is the auto-focusing function which automatically senses the focus
optimized for the analysis software of the JuLI™FL and provides the precise and
rapid counting results.
· The example images of HeLa cells show the reference value for adjusting focus.
Counting
Auto focus
Operation
IMPORTANT!
It is strongly recommended to use Auto focus function after adjusting a focus. If not, it
might be the cause of,
- taking more time for counting (more than 10 seconds)
- incorrect counting results or viability
Focus
by
user in
various
cases
Dark focus
Normal focus
Bright focus
recommend for
JuLI
TM
FL counting
Apply
•
Mix well 10 µL of sample and the 10 µL of 0.4% trypan blue stain
(Bright mode).
•
Place the Fluorescence sample on the scope unit
(Fluorscence mode).
1. Load 10 µL of sample mixture on the counting slide
(side A) using a pipette. The two chambers of the slide are
labeled as “A” and “B” for easy tracking of your samples.
Each chamber is counted separately.
2. Put the counting slide with the slide holder
on the scope unit.
JuLI
TM
FL, Fluorescence Cell History Recorder ©2013 NanoEnTek Inc.
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