4. Choose the monitoring mode among the
Growth rate
, the
Wound healing
and the
Movie only
.
· Movie only means just monitoring cells without calculating the cell confluence
value.
5. After choosing channel 1or channel 2, set movie options
(Choose both channel 1 and channel 2 in case of dual monitoring.).
Edit the
Total time
and the
Interval
to set the total running time
and the time interval.
· The number of total images will be displayed automatically when data value of
“Total Time” and “Interval” are set up.
IMPORTANT!
Warm up scope unit for 1 hour in incubator before making a movie. If not, it might be the
cause of uncertain data or unclear movies.
IMPORTANT!
Cell culture flask should be wetted by the culture media before making a movie.
Operation
Monitoring
·
Minimum interval
Movie only :
30 sec
( 1 or 2 scopes)
Growth rate/ Wound healing :
1 min
(1 scope),
5 min
(2 scopes)
* The 15 minutes time intervals are recommended to prevent the cell
damage when using the fluorescence sample.
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