•
Mix well 10 µL of sample and the 10 µL of 0.4% trypan blue stain
(Bright mode).
•
Place the Fluorescence sample on the scope unit
(Fluorscence mode).
1. Load 10 µL of sample mixture on the counting slide
(side A) using a pipette. The two chambers of the slide are
labeled as “A” and “B” for easy tracking of your samples.
Each chamber is counted separately.
2. Put the counting slide with the slide holder
on the scope unit.
Operation
Counting
Sample-
preparation
34