Molecular Devices SpectraMax L, Руководство пользователя

Страница: 31 / 47

SpectraMax L Luminescence Microplate Reader User Guide
31 0112-0174 C
14. Select the PMT Selections category and then select an option:
Select Default PMT Selection to use all onboard PMTs to do the read.
Select Manual PMT Selection and then select the check box for each PMT to use
for the read.
15. Select the More category.
a. Select the Dark Adapt check box to delay the read for the number of minutes you
enter in the Delay field. This allows the plate autophosphorescence to subside
before the read begins, reducing background.
b. When the instrument has a barcode reader, the following options are available:
Select the Append Barcode to Section Name check box to add the barcode
to the Plate section name in the results.
Select the
File Name Options check box to include the barcode in the file
name and then select one of the following options:
Select Append Barcode to File Name to append the barcode to the file
name.
Select
Overwrite File Name with Barcode to overwrite the file name with
the barcode.
16. After you define the protocol settings, click OK to close the Settings dialog.
17. Create a template, if applicable.
18. Set Data Reduction parameters, if applicable.
19. Set Display options, if applicable.
20. Click .
21. Save the data to a data file and save the settings to a protocol file.
22. After you complete an injector protocol:
a. Reverse the injectors. See Reversing Injectors on page 27 .
b. Place the tubing from the reagent bottles in a container of deionized water.
c. Wash the injectors as recommended by the assay vendor. See Washing Injectors
on page 26 .
Powering Off the Instrument
When you power off the instrument, the shut down process automatically clears the
pumps and fluid lines to prepare the instrument for future use.
The injectors are reversed. This makes sure there is no reagent fluid left in the injector
tubing, valves, and tips of the injection flow path.
The instrument performs an AutoWash to flush wash fluid through the injectors and
fluid lines, if wash bottles are attached.
The plate drawer is placed in the lock position to prevent movement of the drawer if
you relocate the instrument.