SpectraMax L Luminescence Microplate Reader User Guide
27
0112-0174 C
4. Select an option:
Select
Wash From Wash Bottle
to draw the wash liquid from the wash tubing.
Select
Wash From Reagent Bottle
to draw the wash liquid from the reagent
tubing.
5. Select the
Empty Lines After Wash
check box to drain the wash lines.
6. Click
Wash
to flush the injector tubing and tips with fluid. The instrument shuts off
after the wash completes.
Priming Injectors
The Prime Injectors dialog enables you to fill the tubing with reagent, to remove air
bubbles, and prepare the system for injection.
To prime the injectors:
1. In the SoftMax Pro Software, select the Operations tab and click
Prime Injectors
to
display the Prime Injectors dialog.
2. Select a Setting option:
Select
Auto
and then select the check box for the
Pre-Measurement Injectors (P)
and/or the
Measurement Injectors (M)
to automatically prime all P-injectors
and/or M-injectors.
Select
Manual
and then select the check box for each P-injector and each M-
injector to prime.
3. In the
# of Injections Per Injector
field, enter the number of 100-µL injections to do
per prime.
4. In the
P-Inj Prime Speed
field, enter the desired injector speed in µL/second. M-
injector prime speed is fixed at 230 µL/s when you choose to prime the M-injectors.
5. Click
Prime
.
Reversing Injectors
The Reverse Injectors dialog enables you to recover the reagent from the injector tubing.
Reversing the injectors makes sure there is no reagent fluid left in the injector tubing,
valves, and tips of the injection flow path. The reversal process also recovers reagent for
future use.
To reverse the injectors:
1. In the SoftMax Pro Software, select the Operations tab and click
Reverse Injectors
to
display the Reverse Injectors dialog.
2. Select a Setting option:
Select
Auto
and then select the check box for the
Pre-Measurement Injectors (P)
and/or the
Measurement Injectors (M)
to automatically reverse all P-injectors
and/or M-injectors.
Select
Manual
and then select the check box for each P-injector and each M-
injector to reverse.
3. In the
# of Injections Per Injector
field, enter the number of reverse 100-µL injections
to do per injector.
