Chapter 4: Protocols
0112-0174 C
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10. Select the
Timing
category.
For the Endpoint read type, in the
Integration Time
field, enter the number of
seconds required for well measurement.
For the Dual Read type, select
Normal
if the integration times for the read
following the P-injection and the M-injection are the same and enter the number
of seconds required for well measurement or select
Separate
if the integration
times for the read following the P-injection and the M-injection are different.
For the Kinetic read type, enter the integration time, the total run time, and the
interval.
For the Fast Kinetic read type, enter the integration time and the number of
reads per well.
11. Select the
Shake
category.
a. Select the
Before First Read
check box to shake the plate after the injection and
prior to the read.
b. Click the
Shake Type
drop-down.
Select
Classic
to shake the plate along the X-axis, alternating between fast
and slow speed.
Select
Single
to shake the plate along the X-axis at a constant rate.
Select
Dual
to shake the plate in an L pattern.
Select
Orbital
to shake the plate in a circular pattern.
c. In the
Shake Duration
field, enter the number of seconds to shake the plate
between 1 and 999 seconds.
d. In the
Shake Speed
field, enter how fast the plate is to shake in millimeters per
second.
e. In the
Shake Diameter
field, enter the size of the shake diameter.
12. Select the
Injection & Delay
category.
a. Select the
P-Injection
check box to inject using P-injectors and/or select the
M-
Injection
check box to inject using M-injectors. When you select either check
box, an Injection Wells category appears in the category list.
b. For each injection you select, in the
P/M-Injector Volume
field, enter the volume
of fluid to inject.
c. In the
Post Injection Delay
field, enter the number of seconds to delay between
injection and reading.
d. In the
Injection Speed
field, enter the number of µL to dispense per second. The
recommended injection speed for injections is at least 230 µL/s.
e. In the
Shake After Injection
field, enter the number of seconds to shake the
plate after the injection and prior to measurement.
13. Select the
Injection Wells
category to select the wells to receive a P-injection and/or
an M-injection.
a. Select
P Injector
and then select the wells to receive a P–injection. Click a well
again to clear it.
b. Select
M Injector
and then select the wells to receive a M–injection. Click a well
again to clear it.
