Molecular Devices SpectraMax L, Руководство пользователя

Страница: 25 / 47

Chapter 3: Getting Started
0112-0174 C 25
Auto Calculation
This option enables you to use your own assay materials for calibration and have the
software automatically calculate a PMT calibration factor.
To use auto calculation:
1. Remove any plate from the plate drawer.
2. In the SoftMax Pro Software, select the Operations tab and click
Calibration to
display the Calibration dialog.
3. Select the Custom Multi-PMT Normalization Microplate Calibration Method option.
4. Click the Custom Calibration # drop-down and select the custom calibration to
define. You can define two different custom calibrations.
5. Select the Auto-Calculation PMT Calibration Factor option.
6. In the Dark Adapt Delay field, enter the number of seconds to enable the sample to
adapt to the dark interior of the plate drawer before the calibration starts. This allows
the plate autophosphorescence to subside.
7. Click OK .
8. A message displays to tell you to insert a 96-well plate into the instrument with
material of greater than 100k RLU in wells B2, G2, B11, and G11. After you insert the
plate, click
OK .
Manual Set
This option enables you to use your own assay materials for calibration and manually
calculate the PMT calibration factor based on the signal obtained from each PMT. You
can determine these values by reading all or part of a plate that contains your assay
material (should be greater than 100k RLU) and then determining a factor, or multiplier,
for each PMT that equalizes the RLU values for each PMT.
To use manual calculation:
1. In the SoftMax Pro Software, select the Operations tab and click Calibration to
display the Calibration dialog.
2. Select the Custom Multi-PMT Normalization Microplate Calibration Method option.
3. Click the Custom Calibration # drop-down and select the custom calibration to
define. You can define two different custom calibrations.
4. Select the Manual Set PMT Calibration Factor option.
5. Enter the values for each available PMT from your calculations. Available fields are
dependent the instrument configuration.
6. Click OK .