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SkanWasher 400 User Guide

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Chapter 1: Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1

Chapter 2: Installation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

Chapter 3: Parts and Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

Wash Head. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  9
Microplate Lift. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  9
Control Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  10

LED Display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

Instrument Rear Connections . . . . . . . . . . . . . . . . . . . . . . . . 11
Internal Washer Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

Internal Vacuum Source . . . . . . . . . . . . . . . . . . . . . . . . . 13
Internal Pressure Source . . . . . . . . . . . . . . . . . . . . . . . . . 13

Chapter 4: Software Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

Setup Function. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

Edit Program . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Edit Options . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

Chapter 5: Operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

Starting a Wash Program. . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

Chapter 6: Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

Daily Maintenance. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  25
Empty Wash Head Procedure . . . . . . . . . . . . . . . . . . . . . . . .  26
Weekly Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  27
Advanced Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  28

Clogged Probes or Rinse Inlet Channels. . . . . . . . . . . . .  28
Replacing Probes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  31
Microplate Lift Adjustment (Tilt and Vertical)  . . . . . . .  31

Table of Contents

Содержание SkanWasher 400

Страница 1: ...SkanWasher 400 User Guide AN ALL INCLUSIVE AUTOMATIC MICROPLATE WASHER...

Страница 2: ...nd is not to be used in any type of promotion or advertising without permission from MDC All other trademarks or registered trademarks are the property of their respective owners Disclaimer Molecular...

Страница 3: ...Rear Connections 11 Internal Washer Parts 13 Internal Vacuum Source 13 Internal Pressure Source 13 Chapter 4 Software Configuration 15 Setup Function 16 Edit Program 16 Edit Options 20 Chapter 5 Oper...

Страница 4: ...zontal Adjustment 34 Error Messages 35 Appendix A RS 232 Operation A 1 Introduction A 1 Parameters A 1 Communication Protocol A 1 Appendix B Shipment B 1 Appendix C Specifications C 1 Physical C 1 Per...

Страница 5: ...croplate holder and lift 8 Figure 5 SkanWasher 400 9 Figure 6 Control panel LCD and touch keys 10 Figure 7 Rear of the SkanWasher 400 11 Figure 8 Aspirate probe height position 18 Figure 9 Dispense pr...

Страница 6: ...vi SkanWasher 400 User Guide Table 1 Wash Step Selections 19 Table 2 Troubleshooting Chart 33 Table A 1 Character Commands Recognized A 2 Table A 2 Character Replies Sent A 3 Tables...

Страница 7: ...ing is an essential component of the assay The SkanWasher 400 is designed to wash 96 well microplates The process of setting up wash parameters is flexible and allows you to modify or choose the follo...

Страница 8: ...6 Detailed procedures will be listed for care and maintenance of instrument Chapter 7 Troubleshooting tips error messages and advanced troubleshoot ing will be outlined Appendix This section will incl...

Страница 9: ...e reservoir Sensors can be disabled through the Setup menu Edit Options Consult page 20 for procedure Serial Number The serial number of the unit can be found on the back panel of the instrument Packa...

Страница 10: ......

Страница 11: ...of the instrument Plug the power cord into the main power outlet Step 4 Connect Waste Reservoir refer to Figure 1 Connect the outlet tubing line red from the waste reservoir to the Outlet port on the...

Страница 12: ...reservoir to the Pressure port on the back of the instrument The large connector at the end of the tubing will be used for emptying of the head and will not be connected during normal operation CAUTIO...

Страница 13: ...the instrument Figure 2 Wash and rinse liquid reservoirs IMPORTANT Only one reservoir bottle can be connected at one time for either wash buffer or rinse solution since the unit has only one inlet por...

Страница 14: ...type round or flat bottomed wells in use by selecting the correct printed pattern on the side of the microplate holder See Figure 3 Push the plate holder onto the two rods on the microplate lift see...

Страница 15: ...of the wash program The vertical velocity of the lift is fixed except during the aspirate function the longer the aspirate time the slower the lift moves upward toward the aspirate probes This creates...

Страница 16: ...s Setup Program Enter and the up and down arrow keys are used to select and enter parameters when programming the SkanWasher 400 When the Setup or Program key is pressed its corresponding LED will lig...

Страница 17: ...re three LEDs that light up indicating various functions Setup LED will light when user is in setup mode Program LED will light when user is in program mode Ready LED will light when unit is ready for...

Страница 18: ...Located on the back panel of the unit This contains the main power receptacle which includes the power switch and fuses A voltage switch located below the power cord receptacle allows the user to set...

Страница 19: ...iminating the need for gravity feed The pressure level can be adjusted between 0 0 and 0 6 bar approximately 9 psi The air pressure is supplied through the small quick couplings on the rear side of th...

Страница 20: ......

Страница 21: ...robe above the bottom of the well during aspiration can be varied The amount of fluid kept in the well can be adjusted Dispense probe height position in the well during washing can be set Up to 16 was...

Страница 22: ...n pressing the Setup key the Setup Main menu will display two options Edit Program A wash program can be edited or created using this selection Edit Options Instrument parameters can be viewed and sen...

Страница 23: ...o adjust the inlet wash liquid flow To disable the pressure pump select 0 0 bar For normal wash select 0 25 bar approximately 4 psi Volume Adjust Adjustment can be made to fine tune the dispensed vol...

Страница 24: ...icroplate in lift and press Enter to raise microplate to probes Use up down arrow buttons to set at correct height See Figure 9 Total adjustable distance is 6 mm Wash Program 1 16 Up to 16 wash steps...

Страница 25: ...t volume of wash from 100 900 l Wash time Set time from 0 1 to 10 seconds Aspirate Set aspiration time from 2 0 10 seconds Lift velocity is varied with selected aspirate time The lift velocity is auto...

Страница 26: ...r On Off Read Sensor Sensor On Off The sensors can be enabled or disabled by selecting this option Step 1 Press Setup on the Control Panel to enter the Setup Main Menu Step 2 Press Down arrow key to s...

Страница 27: ...T When sensors disabled all three safety features will be disengaged Air Pressure Value is actual air pressure on internal pressure source displayed in bar Changes can be made through software program...

Страница 28: ......

Страница 29: ...lder Check pattern on the side of microplate holder to verify that the holder is in the correct plate configuration Press Rinse key to enter prime program Press Start Repeat 3 times Step 5 Choose Wash...

Страница 30: ...late Holder Check pattern on the side of microplate holder to verify that the holder is in the correct plate configuration Step 7 Press Start key Step 8 Repeat Step 5 8 to wash additional plates Step...

Страница 31: ...r Step 3 Verify waste tubing is connected to outlet port Step 4 Insert a clean microplate into the microplate holder Step 5 Press the Rinse key to enter the Rinse Prime mode Step 6 Press the Rinse key...

Страница 32: ...p 2 Disconnect liquid line from the Inlet port on the instrument s wash head Step 3 Connect large connector from pressure tubing to Inlet port Step 4 Verify waste tubing is connected to outlet port St...

Страница 33: ...ogram will rinse for 5 seconds and then soak the probe tips for 5 seconds This cycle is repeated 3 times Step 6 Soak Probes Press Stop key to pause when instrument is in first soak cycle of Rinse prog...

Страница 34: ...cles Press the Rinse key to enter the Rinse Prime mode Press the Start key a second time to start the prime program Repeat 3 times Step 7 Run a wash program to verify that particles are removed Step 8...

Страница 35: ...se Prime mode Press the Start key a second time to start the prime program Water will flush out through the holes in the back of the wash head Repeat if indicated Step 7 Reseat inlet channel seal bloc...

Страница 36: ...et access to the wash head outlet channel see Figure 11 Remove two hex wrench screws using the hex wrenches available in the tool kit Pull out the wash head block to expose the outlet channels Step 2...

Страница 37: ...Loctite Threadlocker Green does not set up as quickly as QuickTite Microplate Lift Adjustment Tilt and Vertical The microplate lift is factory adjusted for optimal operation and should nor mally not n...

Страница 38: ...Tilt Adjustment Screw to tilt the placeholder upward loosen this screw to tilt the plateholder down Figure 12 Microplate lift adjustment CAUTION Don t use force to tilt the plate holder If you need t...

Страница 39: ...o power to instrument instrument will not turn on Check that power cord is plugged in Check power active to source outlet Check voltage selection switch Check power supply Air in lines Prime lines inc...

Страница 40: ...djust Programs not retained in instrument memory Battery on main board may be low battery life is from three to five years Test the battery strength to see if it needs to be replaced Strength should b...

Страница 41: ...e prime The actual air pressure is not equal to the set air pressure value Low Probably a leak Check that all reservoir caps are tightened High Due to changed air pressure setting The previ ously used...

Страница 42: ......

Страница 43: ...arity None Stop bits 1 The SkanWasher 400 comes with an RS 232 cable having 9 pin male female connectors The communication protocol is designed for single character commands No data handshake control...

Страница 44: ...ished Reply G 47h E 45h 21h S s 57h 73h Stop interrupt the current action of the Skan Washer This command performs the same function as pressing the Stop key on the control panel The Stop command must...

Страница 45: ...ram that was not defined The SkanWasher 400 will only accept a select program com mand if the wash program number between 1 and 8 has been defined 1 31h 2 32h 8 38h The wash program now selected 3Fh C...

Страница 46: ...a microplate is present will be verified prior to initiating the wash Start command accepted Reply G Not accepted due to sensor alarm Reply E When wash sequence finished Reply S s 57h 73h Stop interr...

Страница 47: ...ls are used use appropriate decontami nate solution for each material Step 2 Perform Empty Head procedure to empty dispense head of all fluid Step 3 Clean exterior surface of instrument with alcohol f...

Страница 48: ......

Страница 49: ...brane pump Microplate lift Step motor driven Performance Aspirate 2 l average residual volume well Dispense Precision 2 CV Accuracy 3 Internal volume Wash head prime volume 35 ml Well flow rate water...

Страница 50: ......

Страница 51: ...umber ____________________ Method of Decontamination ______________________________________ _____________________________________________________________ ______________________________________________...

Страница 52: ......

Страница 53: ...Rinse 2 0 sec ____ l 4 Wash Step Aspirate Wash Dispense Soak Rinse 1 5 sec ____ l 5 Wash Step Aspirate Wash Dispense Soak Rinse 2 0 sec ____ l 6 Wash Step Aspirate Wash Dispense Soak Rinse 1 5 sec __...

Страница 54: ...2 0 sec ____ l 4 Wash Step Aspirate Wash Dispense Soak Rinse _____ sec 250 l 5 Wash Step Aspirate Wash Dispense Soak Rinse 2 0 sec ____ l 6 Wash Step Aspirate Wash Dispense Soak Rinse _____ sec 250 l...

Страница 55: ...p Aspirate Wash Dispense Soak Rinse 2 0 sec ____ l 4 Wash Step Aspirate Wash Dispense Soak Rinse _____ sec 200 l 5 Wash Step Aspirate Wash Dispense Soak Rinse 5 0 sec ____ l 6 Wash Step Aspirate Wash...

Страница 56: ...ate Wash Dispense Soak Rinse _____ sec ____ l 4 Wash Step Aspirate Wash Dispense Soak Rinse _____ sec ____ l 5 Wash Step Aspirate Wash Dispense Soak Rinse _____ sec ____ l 6 Wash Step Aspirate Wash Di...

Страница 57: ...se _____ sec ____ l 6 Wash Step Aspirate Wash Dispense Soak Rinse _____ sec ____ l 7 Wash Step Aspirate Wash Dispense Soak Rinse _____ sec ____ l 8 Wash Step Aspirate Wash Dispense Soak Rinse _____ se...

Страница 58: ...se _____ sec ____ l 6 Wash Step Aspirate Wash Dispense Soak Rinse _____ sec ____ l 7 Wash Step Aspirate Wash Dispense Soak Rinse _____ sec ____ l 8 Wash Step Aspirate Wash Dispense Soak Rinse _____ se...

Страница 59: ...vices Corporation at the telephone number or address below to locate the nearest service facility At the time of requesting warranty service you must present proof of purchase documentation which incl...

Страница 60: ...this agreement is for the sole purpose of identifying them Any such description is not part of the basis of the bargain and does not constitute a warranty that the products shall conform to that desc...

Страница 61: ...5 27 B 1 D 1 Dispense Probe Position 18 Dispense Volume 19 E Edit Options 16 20 Edit Program 16 Empty Wash Head Procedure 26 Enter key 10 Error messages 35 H Home position microplate lift 9 I Installa...

Страница 62: ...al number 3 Set Aspirate Position 17 Set Dispense Position 18 Setup function 16 Setup key 10 Setup LED 11 Shipment B 1 Software 15 Specifications C 1 Start key 10 Stop key 10 T Tilt adjustment screw 3...

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