Molecular Devices QPix 420, Руководство пользователя

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Chapter 7: Blue-White Picking Processes
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3. For a fluorescent image, adjust the Intensity Measure as shown in the histogram. This
option is available only for instruments with a fluorescence imaging module.
Mean Intensity is the average fluorescence of the detected colony (the fluorescence of
all the pixels within the colony perimeter divided by the number of pixels within the
colony perimeter).
Median Intensity is the middle fluorescence value of all the pixels within the detected
colony perimeter.
Geometric Intensity is the geometric intensity of the detected colony or the
fluorescence of all the pixels within the colony perimeter.
Centre Mean Intensity is the average fluorescence value of the middle 9 pixels within
the detected colony perimeter.
Select Greater Than, Less Than, or Between and enter a value in the field to define the
fluorescence intensity threshold. For Between, enter two values to define the range.
4. Change the pickable property of individual objects.
To define an object as pickable, right-click the object and select Pick Item to display the
object in green.
To define an object as unpickable, right-click the object and select Discard Item to
display the object in blue.
5. In the Limit Colonies field, enter the maximum number of colonies to pick from each
receptacle.
Note: If a limit was previously set for the maximum number of colonies in the Source
window, the Limit Colonies option is not available in the Feature Selection window.
See
on page 45 .
The Total Feature Count field displays the total number of pickable objects in the
receptacle.
6. Select the Feature Counts tab to view the number of found features in a source receptacle.
The barcode or identifier for the source receptacle displays, along with the number of found
colonies and the number of colonies to pick as determined by the selection criteria.
To save the data in .csv format, right-click and then click Export.