Molecular Devices QPix 420, Руководство пользователя

Страница: 13 / 225

5031496 G 13
Chapter 1: Introduction
With the QPix® 420 Colony Picking System from Molecular Devices, you can control selective
microbial colony picking. Multiple users can select and collect microbial colonies from various
receptacles using the QPix 420 system Instrument with the QPix 420 Colony Picking Software.
Before operating the instrument or performing maintenance operations, make sure you are
familiar with the safety information in this guide. See
Safety Information on page 7 .
For research use only. Not for use in diagnostic procedures.
Functionality of the QPix 420 System
The QPix 420 system is part of the QPix 400 series. The QPix 420 system offers a range of
features to accommodate various requirements for picking of microbial colonies including
multiple imaging modes, organism-specific pins, agar height sensor, and specific algorithms to
detect variable biological samples. Other functions include sample rearraying, replication,
organism-specific pins, color screening, and gridding. See
Replacement Parts and Optional
Extras on page 215 .
The QPix 420 system is useful for working with applications such as protein engineering,
protein evolution, directed or enzyme evolution, protein expression, transformation, and sub-
clone management.
The QPix 420 system is available with white light or fluorescent imaging. Color filters, such as
for blue-white screening, are available in combination with white light imaging.
Multiple fluorescent filters ensure compatibility with a wide range of fluorescent cloning dyes
and proteins and enable the system to reveal unique information about individual colonies.
Fluorescent imaging is available as an option.
After colonies are located using a CCD camera, they are picked at high speed from source
receptacles and then inoculated into pre-filled 96-well or 384-well plates. Colonies of interest
can be rearrayed from a library into new plates.
To optimize these systems, Molecular Devices has developed a range of plastic consumables
for use with the instrument. The destination plate bed enables versatile use of shallow,
standard, and deep-well plates in various combinations. Organism-specific pins can be ordered
to optimize the efficiency of transfer.
You can log all your processes such as picking, replicating, and re-arraying with the QPix 420
system Colony Picking. The software lets you tag important samples to enhance the history,
location, and extra details of sample-specific data.
1