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Chapter 13: Replication Processes

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Running Replication Processes

After a replication routine has been configured, you can run the process on the instrument. If
you have not configured the replication routine to run, you must create a new replication routine
or edit an existing routine.

Note: Before you running a picking process, it is important that you do the cleaning and
set up procedures in

Preparing to Run Processes on page 27

.

Some steps that are included in these procedures might not be available, depending on the
features included with the instrument and license.

To run a replication routine:

1. Open the Library Replication, Library Compression, or Library Expansion window.

2. Select the replication routine you want to run.

To not make changes to the routine, select the Skip Steps check box before you click Next.

3. Review the settings for the routine.
4. In the Settings Summary window, click Next.

5. In the Continue or Save New Routine dialog or the Save Changes to Routine dialog, select

whether or not to save the routine before continuing with the replication process.
If you create a new routine, the Continue or Save New Routine dialog displays.

To save the settings for the routine before continuing, click Save Routine, enter a
Name and a short Description for the routine, and then click Save.
To continue without saving the settings for the routine, click Routine Without Saving
and then click OK.

If you edit a routine, the Save Changes to Routine dialog displays.

To save the settings for the routine before continuing, click Save.
To save the settings as a new routine without changing the existing routine, click Save
As, enter a Name and a short Description for the routine, and then click Save.
To continue without saving the settings for the routine, click No.

6. Make sure that the instrument door is closed.

7. Click Next to run the replication routine.

8. The Library Replication Progress, Library Compression Progress, or Library Expansion

Progress window displays.

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Содержание QPix 420

Страница 1: ...5031496 G August 2022 QPix 420 Colony Picking System User Guide...

Страница 2: ...r license to use or permit others to use such manufacturers and or their product names as trademarks Each product is shipped with documentation stating specifications and other technical information M...

Страница 3: ...the QPix 420 System 13 Chapter 2 QPix 420 System Instrument Overview 15 Front Panel Controls and Display 16 Instrument Connections 17 Compressed Air Supply 18 Using Barcodes 18 Computer System Requir...

Страница 4: ...Blue White Regional Picking Processes 120 Running Blue White Regional Picking Processes 128 Chapter 11 Control Plate Creation Processes 139 Creating and Editing Control Plate Creation Processes 140 R...

Страница 5: ...205 Moving the Instrument 206 Adding a New User to the SQL Server Database 207 Running the Software as an Administrator 207 Resetting the Path to the SQL Server 207 Obtaining Support 209 Chapter 18 Tr...

Страница 6: ...QPix 420 Colony Picking System User Guide 6 5031496 G...

Страница 7: ...of other types of hazards such as biohazard electrical or laser safety warnings as are described in the text of the warning When warnings and cautions are displayed in this guide be careful to follow...

Страница 8: ...All safety instructions must be read and understood before proceeding with the installation maintenance and servicing of all modules Power off the instrument and disconnect the power cord before you...

Страница 9: ...als being used Electrical Safety To prevent electrically related injuries and property damage inspect all electrical equipment before use and immediately report all electrical deficiencies Contact Mol...

Страница 10: ...V light to prevent the risk of damage from UV radiation Magnet Safety Persons with external or implanted medical devices need to evaluate the risks related to these devices before entering an area whe...

Страница 11: ...ea must be clearly marked with an applicable biohazard sign WARNING Never use the instrument in an environment where potentially damaging liquids or gases are present Moving Parts Safety To prevent in...

Страница 12: ...page 11 for details Avoid spilling liquids on the system Fluid spilled into internal components creates a potential shock hazard Wipe up all spills immediately Do not operate the system if internal c...

Страница 13: ...as protein engineering protein evolution directed or enzyme evolution protein expression transformation and sub clone management The QPix 420 system is available with white light or fluorescent imagin...

Страница 14: ...uses an LED based light source and preset filter pairs for selecting fluorescence excitation and emission wavelengths and a sensitive monochrome CCD camera for image acquisition The fluorescence head...

Страница 15: ...you run a process The door prevents UV light from passing through during operation The bed of the instrument contains source and destination receptacle positions The bed also contains wash baths whic...

Страница 16: ...em Description 1 LCD indicator panel 2 START button 3 STOP button 4 Emergency Stop button 5 Power is on 6 Air pressure is low 7 Air pressure is correct 8 The instrument is running a process not applic...

Страница 17: ...ht side of the instrument 1 2 3 4 5 6 Item Description 1 Power Inlet Instrument Mains 2 Power Outlets Computer and Monitor 3 Ethernet port 4 Compressed air inlet 5 Fuse carrier Instrument Mains 6 Fuse...

Страница 18: ...h the filter regulator inlet Use only 6 mm OD polyurethane pneumatic hose rated for more than 100 psi to connect to the air inlet CAUTION The filter regulator is factory set to 0 69 MPa 100 psi at 60...

Страница 19: ...10 64 bit Windows 7 32 bit Memory 4 GB RAM Data Connection 10 100 Ethernet port Camera Connection USB 2 0 port Minimum Computer System Requirements CAUTION Do not replace the computer operating the sy...

Страница 20: ...QPix 420 Colony Picking System User Guide 20 5031496 G...

Страница 21: ...e window below the menus New Process gives you a full set of options for creating and managing processes and system maintenance See New Process Options on page 23 Templates displays a list of saved te...

Страница 22: ...Recent Processes displays a list of recently opened processes Switch User can be used in a multiple user environment to log off the current user so that a different user can log on Exit closes the Na...

Страница 23: ...m receptacles using the regional process See Blue White Regional Picking Processes on page 119 Control Plate Creation creates a batch of plates containing control samples These can be created by picki...

Страница 24: ...s process whenever the head is returned to the actuator or whenever the actuator is hit as this can have a negative effect on picking precision See Aligning the Camera on page 202 Instrument Utilities...

Страница 25: ...is from 0 69 MPa to 0 83 MPa 100 psi to 120 psi If you are using a standalone air compressor confirm that the power cord for the compressor is properly connected and that power to the power outlet is...

Страница 26: ...the computer to turn off 3 Push the Stop button on the front panel to switch off the instrument 4 Turn off the power at the mains or disconnect the power cord to the instrument 5 Turn off the air sou...

Страница 27: ...2 7 Prepare plates QTrays OmniTrays or Petri dishes based on the requirements for your application 8 Select and run the desired process For information about selecting defining and running processes s...

Страница 28: ...es use the software Change Head process to safely move the actuator To change the head 1 From the Navigation window under Utility Processes double click the Instrument Utilities icon 2 In the Instrume...

Страница 29: ...or whenever it needs cleaning wipe the exterior of the head with a cloth using 70 ethanol To store the head slide it into its metal cover with the pins facing inward and then secure it in place with t...

Страница 30: ...QPix 420 Colony Picking System User Guide 30 5031496 G...

Страница 31: ...lly clean the instrument interior To run a Sanitise process 1 From the Navigation window under Utility Processes double click Instrument Utilities 2 In the Instrument Utilities window double click San...

Страница 32: ...ow select a Sanitise Profile type the duration in seconds for the UV light to remain on The default duration is 600 seconds 10 minutes 4 Confirm that the bed is clear and that the door is closed Tip T...

Страница 33: ...ycles can contain one or more wash bath steps that can be created in the Manage Sanitise Profiles window Tip You cannot edit Sanitise profiles when setting up a routine for a process While you are wor...

Страница 34: ...file dialog in the Name field enter a short descriptive name for the profile 4 Select one of the following options for the wash profile Select Single Stage to use the same wash cycle before and during...

Страница 35: ...tility Processes double click Manage Sanitise Profiles 2 In the Manage Sanitise Profiles window in the Profiles table click the profile you want to edit If the profile is a Single Stage profile the St...

Страница 36: ...conds list enter the number of seconds to dry the pins with the halogen dryer 7 From the Wait After Drying seconds list enter the number of seconds to wait before moving on to the next step or ending...

Страница 37: ...1 From the Navigation window under Utility Processes double click the Manage Sanitise Profiles icon 2 In the Manage Sanitise Profiles window in the Profiles table click the profile you want to delete...

Страница 38: ...QPix 420 Colony Picking System User Guide 38 5031496 G...

Страница 39: ...containing control samples These can be created by picking colonies from source receptacles into destination wells See Control Plate Creation Processes on page 139 Create New Regional Tray adds new pl...

Страница 40: ...ow under Picking Processes double click Picking 2 In the Picking window click Picking Type to view the picking type options Select White Light to use only white light to illuminate and identify the co...

Страница 41: ...he routine is of the same type as the one you are creating then the imported routine displays in the Select Routine list Exporting Routines To export a routine click Export and then navigate to the lo...

Страница 42: ...ow the system to automatically assign a valid random barcode and continue the routine 3 Use the Validation Barcode list to define the barcodes for source receptacles If the scanned barcode on a source...

Страница 43: ...om which to import the identifiers To remove an identifier from the list click the identifier in the list and then click Remove To generate a defined list of identifiers for the source receptacles cli...

Страница 44: ...py check box and from the Select Copy Microplate list select the plate type to use for a duplicate copy of the destination plate This sends the picking head to inoculate a second plate without returni...

Страница 45: ...sferring the picked colonies to the destination plate For the fastest transfer rate select All to use all available picking pins to pick colonies before inoculating the destination plate For smaller p...

Страница 46: ...vailable 5 In the Picking Depth Into Agar mm field enter a value in millimeters to define the depth that the pins are to be inserted in the agar for picking 6 Select the Limit Max Number of Features P...

Страница 47: ...r the front of the instrument 3 Open the front door 4 Install the picking head To remove an installed head unscrew and remove the thumbscrew and washer on the left that secures the head to the actuato...

Страница 48: ...nes on page 40 If you do not need to make changes to the routine select the Skip Steps check box before clicking Next 3 Review the configured settings for the routine See Viewing the Settings Summary...

Страница 49: ...nies are detected adjust the test image in this window using the Exposure and Gain settings the Detection settings the Display settings the Debris settings and the Depth settings Each detected feature...

Страница 50: ...ain settings click Grab Image to apply the new settings to the test image 3 Select the Detection tab 4 Select the Use Auto Thresholding check box to have the software automatically detect the colonies...

Страница 51: ...shold Each Frame check box again and enter the value from the Threshold field into the High Limit field 7 Select the Invert Image check box to make dark areas bright and bright areas dark Tip This is...

Страница 52: ...ight for a long pin head to descend into the source agar Note Further refinement for colony detection can be done on a higher resolution image in the Feature Selection window 15 Click Next to go to th...

Страница 53: ...o expose the sample to light when acquiring the image Adjust the Gain to increase the image signal without changing the light exposure After you adjust the Exposure or Gain settings click Grab Image t...

Страница 54: ...t without a yellow ring in the test image is excluded from becoming a pickable object Drag the Zoom slider below the image to get a closer look at the image and drag the Contrast slider to change the...

Страница 55: ...ou want to change When clicked as an override the yellow marked pickable objects change to blue and become unpickable To undo the selection right click over the image map and select Pick Item The obje...

Страница 56: ...erimeter divided by the area of the colony so that irregular shaped colonies are closer to 0 and colonies that are more of a perfect circle are closer to 1 A colony equal to or less than 0 65 is not p...

Страница 57: ...e intensity threshold For Between enter two values to define the range 4 Change the pickable property of individual objects To define an object as pickable right click the object and select Pick Item...

Страница 58: ...ocess If you create a new routine the Continue or Save New Routine dialog displays To save the settings for the routine before you continue click Save Routine enter a Name and a short Description for...

Страница 59: ...ct Make Copy for the routine Destination Barcode is the barcode of the destination receptacle that is currently being inoculated Destination Offset is the reference well for where the pins are lowered...

Страница 60: ...nstrument deck 5 Close the instrument door 6 Click Next to continue running the picking routine If you click Continue With Image Review the system takes a white light test image of the source receptac...

Страница 61: ...r the front of the instrument 3 Open the front door 4 Install the picking head To remove an installed head unscrew and remove the thumbscrew and washer on the left that secures the head to the actuato...

Страница 62: ...QPix 420 Colony Picking System User Guide 62 5031496 G...

Страница 63: ...Note Before running a picking process it is important that you do the cleaning and set up procedures in Preparing to Run Processes on page 27 If this is your first picking process you must edit or cr...

Страница 64: ...page 70 Changing the Picking Head on page 70 Note Some steps that are included in these procedures might not be available depending on the features included with the instrument and license Opening the...

Страница 65: ...Routines Before you can import a routine you must export the routine from the other computer in xml format and then save the export file on the system computer To import the xml file click Import and...

Страница 66: ...ode Note If the system fails to identify a barcode for the source receptacle the routine ends since the source requires a valid barcode Click Auto Generate to allow the system to automatically assign...

Страница 67: ...To import identifiers for the source receptacles from a text or csv file click Import and then select the file from which to import the identifiers To remove an identifier from the list click the iden...

Страница 68: ...Click Fill All Microplates to fill every selected well of a destination plate before starting a new destination plate Click New Microplate for Each Position to start a new destination plate whenever...

Страница 69: ...rofiles are not suitable for the picking routine exit the picking process and create a new Sanitise profile See Creating and Editing Sanitise Profiles on page 33 5 Click Next to define options for the...

Страница 70: ...ick Move to Load Position to move the picking head arm to the loading position 2 When the message displays make sure that the bed is clear of obstructions and that the door is closed and then click OK...

Страница 71: ...the picking routine to run See Selecting Blue White Picking Routines on page 64 If you do not need to make changes to the routine select the Skip Steps check box before clicking Next 3 Review the sett...

Страница 72: ...destination plates To make sure that only blue or white colonies are detected adjust the test image in this window using the Exposure and Gain settings the Detection settings the Display settings and...

Страница 73: ...conds from 1 to 2000 to expose the sample to light when acquiring the image Adjust the Gain to increase the image signal without changing the light exposure Tip Molecular Devices recommends not changi...

Страница 74: ...Range that starts near the left side of the histogram and ends to the left of the large spike that represents the largest area which is the agar To manually select White Colonies increase the minimum...

Страница 75: ...or colony detection can be done on a higher resolution image in the Feature Selection window 10 Click Next to go to the next step Selecting Blue White Colonies for Picking After you adjust and refine...

Страница 76: ...he objects and the background To save the image in bmp jpg or png format click Export Image To select a smaller region of interest ROI in the image draw a polygon around the region To draw a polygon c...

Страница 77: ...change When clicked as an override the yellow marked pickable objects change to blue and become unpickable To undo your individual selection right click over the image map and select Pick Item The ob...

Страница 78: ...perimeter divided by the area of the colony so that irregular shaped colonies are closer to 0 and colonies that are more of a perfect circle are closer to 1 A colony equal to or less than 0 65 is not...

Страница 79: ...escence intensity threshold For Between enter two values to define the range 4 Change the pickable property of individual objects To define an object as pickable right click the object and select Pick...

Страница 80: ...ocess If you create a new routine the Continue or Save New Routine dialog displays To save the settings for the routine before you continue click Save Routine enter a Name and a short Description for...

Страница 81: ...you select Make Copy for the routine Destination Barcode is the barcode of the destination receptacle that is currently being inoculated Destination Offset is the reference well for where the pins are...

Страница 82: ...ose the instrument door 6 Click Next to continue running the picking routine If you click Continue With Image Review the system takes a white light test image of the source receptacles and the Test Im...

Страница 83: ...These can be created by picking colonies from source receptacles into destination wells See Control Plate Creation Processes on page 139 Create New Regional Tray adds new plate source definitions to...

Страница 84: ...ncluded in these procedures might not be available depending on the features included with the instrument and license Opening the Zone of Inhibition Detection Picking Window To open the Zone of Inhibi...

Страница 85: ...atabase Importing Routines Before you can import a routine you must export the routine from the other computer in xml format and then save the export file on the system computer To import the xml file...

Страница 86: ...fails to identify a barcode for the source receptacle the routine ends since the source requires a valid barcode Click Auto Generate to allow the system to automatically assign a valid random barcode...

Страница 87: ...k Insert To import identifiers for the source receptacles from a text or csv file click Import and then select the file from which to import the identifiers To remove an identifier from the list click...

Страница 88: ...es By Columns or By Rows 5 Define the Deposit Strategy using one of the following options To fill every selected well of a destination plate before starting a new destination plate click Fill All Micr...

Страница 89: ...are not suitable for the picking routine exit the picking process and create a new Sanitise profile See Creating and Editing Sanitise Profiles on page 33 5 To define the source receptacle options cli...

Страница 90: ...ick Move to Load Position to move the picking head arm to the loading position 2 When the message displays make sure that the bed is clear of obstructions and that the door is closed and then click OK...

Страница 91: ...ing Window on page 84 2 Select the picking routine to run See Selecting Zone of Inhibition Detection Picking Routines on page 84 If you do not need to make changes to the routine select the Skip Steps...

Страница 92: ...te Light Test Image In the Test Image window adjust the exposure level to best view the foreground image from the background using the Exposure and Gain settings To view the pixel intensity values in...

Страница 93: ...ncrease the image signal without changing the light exposure Tip Increase the Exposure until the secondary layer is highly exposed and appears very white After you adjust the Exposure or Gain settings...

Страница 94: ...lony to display the properties of that colony If a colony has not been selected as pickable the reason for the exclusion shows in red text Drag the Zoom slider below the image to get a closer look at...

Страница 95: ...he gallery images to show the colonies that meet or fail to meet the criteria To exit the gallery click Return to Image Switch between the two views to define the pickable colonies To change the selec...

Страница 96: ...colony is The value is a ratio of the longest diameter divided by the shortest diameter so oval shaped colonies are closer to 0 and round are closer to 1 The system default value is that a colony equ...

Страница 97: ...colonies some shading for clearer visualization Select the Display Proximity Indicators check box to display connecting red lines between a detected colony and its closest neighbor Select the Shade Ex...

Страница 98: ...ly doing such as Pick when the system is picking To turn the light table on or off click Light Table On or Light Table Off To safely pause the routine click Pause After all the colonies are picked fro...

Страница 99: ...These can be created by picking colonies from source receptacles into destination wells See Control Plate Creation Processes on page 139 Create New Regional Tray adds new plate source definitions to t...

Страница 100: ...cesses double click the Regional Picking icon 2 In the Regional Picking window click Picking Type to view the picking type options Select White Light to use only white light to illuminate and identify...

Страница 101: ...utines Before you can import a routine you must export the routine from the other computer in xml format and then save the export file on the system computer To import the xml file click Import and th...

Страница 102: ...when a barcode cannot be read Click Manual Prompt to open a dialog where you can enter a barcode or name for the receptacle Click Skip Receptacle to ignore the receptacle and scan the next receptacle...

Страница 103: ...o import identifiers for the source receptacles from a text or csv file click Import and then select the file from which to import the identifiers To remove an identifier from the list click the ident...

Страница 104: ...ur system you might have only one option in the Holder list in which case the Receptacle option is preset and cannot be changed If the type of source receptacle that you need is not listed contact Mol...

Страница 105: ...e With the Reserve Wells for Region check box selected select the Skip Region If Picking Count is Zero check box to skip regions that have a picking count of zero 2 Select the Deposit Order to deposit...

Страница 106: ...the Wash Between Partial Inoculation check box Clear this check box to wait until all pins are used for picking and inoculation before running the wash routine 3 In the Destination field enter the di...

Страница 107: ...move the actuator into position near the front of the instrument 3 Open the front door 4 Install the picking head To remove an installed head unscrew and remove the thumbscrew and washer on the left...

Страница 108: ...s on page 100 If you do not need to make changes to the routine select the Skip Steps check box before clicking Next 3 Review the configured settings for the routine See Viewing the Settings Summary o...

Страница 109: ...the region to view in the receptacle image below the list The region is in red 2 Under Acquisition adjust the Exposure and Gain settings In the Exposure ms field enter the number of milliseconds from...

Страница 110: ...make dark areas bright and bright areas dark 8 Select the Subtract Background check box to have the background become nearly black 9 Select the Display tab 10 Select the method for viewing the detecte...

Страница 111: ...tion image and then displays the Feature Selection window If you are running a routine for white light and fluorescent imaging the Fluorescent Test Image window displays Adjusting the Fluorescent Test...

Страница 112: ...tings 3 Click Next to capture and process a higher resolution image and then open the Feature Selection window Selecting Colonies for Regional Picking After you adjust and refine test images the syste...

Страница 113: ...the image and drag the Contrast slider to change the contrast between the objects and the background To save the image in bmp jpg or png format click Export Image To change the selection of the syste...

Страница 114: ...tions click the Remove User Selections icon to display the Remove User Selections dialog Click Yes If you run a routine for white light and fluorescent imaging two tabs are available The White Light t...

Страница 115: ...qual to or greater than the value in this field is not picked Min Proximity mm sets the distance between colonies to be picked so that when picking one colony a different adjacent colony is not picked...

Страница 116: ...gion on the receptacle image If you do not place a limit on the number of colonies to pick for each region all the numbers display in green If you create a limit on detectable colonies the number disp...

Страница 117: ...ptacles and delivered to the destination plates the Finished Picking Current Batch dialog displays If there are no more source receptacles to be picked click Finish Picking and then click OK To contin...

Страница 118: ...vities related to the source and destination receptacles click Details To save the detailed information in csv format click Export To close the picking details and return to the Regional Picking Summa...

Страница 119: ...Note Before running a picking process it is important that you do the cleaning and set up procedures in Preparing to Run Processes on page 27 If this is the first picking process you must edit or cre...

Страница 120: ...on page 122 Setting Destination Plate Options on page 124 Selecting the Regional Picking Source on page 124 on page 124 on page 125 Viewing the Settings Summary on page 126 After creating the routine...

Страница 121: ...h the type of routine that you are creating are included in the list For example a white light and fluorescent imaging routine is not included in the Select Routine list if you are creating a White Li...

Страница 122: ...re you can enter a barcode or name for the receptacle Click Skip Receptacle to ignore the receptacle and scan the next receptacle for a barcode Note If the system fails to identify a barcode for the s...

Страница 123: ...sert To import identifiers for the source receptacles from a text or csv file click Import and then select the file from which to import the identifiers To remove an identifier from the list click the...

Страница 124: ...he system you might have only one option in the Holder list in which case the Receptacle option is preset and cannot be changed If the type of source receptacle that you need is not listed contact Mol...

Страница 125: ...destination plate With the Reserve Wells for Region check box selected select the Skip Region If Picking Count is Zero check box to skip regions that have a picking count of zero 2 Select the Deposit...

Страница 126: ...Between Partial Inoculation check box Clear this check box to wait until all pins are used for picking and inoculation before running the wash routine 3 In the Destination field enter a value for the...

Страница 127: ...OK to move the actuator into position near the front of the instrument 3 Open the front door 4 Install the picking head To remove an installed head unscrew and remove the thumbscrew and washer on the...

Страница 128: ...White Regional Picking Routines on page 121 If you do not need to make changes to the routine select the Skip Steps check box before you click Next 3 Review the configured settings for the routine Se...

Страница 129: ...es Adjusting the Blue White Regional White Light Test Image In the Test Image window all detected features are viewed as potential colonies to pick for inoculation of the destination plates To make su...

Страница 130: ...apply the settings to the test image 3 Select the Detection tab 4 In the Threshold intensity histogram click and drag the red slider line to the right of the large spike to view the White colonies as...

Страница 131: ...mage Click Image Only to display the detected colonies in white with yellow rings and a gray background Click Image and Overlay to display the detected colonies in green with yellow rings and a gray b...

Страница 132: ...been selected as pickable the reason for the exclusion shows in red text Note During image processing each object with no yellow ring in the test image is excluded from becoming a pickable object Dra...

Страница 133: ...map that you want to change When clicked as an override the yellow marked pickable objects change to blue and become unpickable To undo the selection right click over the image map and select Pick It...

Страница 134: ...5 and is not picked Axis Ratio measures how oval the colony is The value is a ratio of the longest diameter divided by the shortest diameter so oval shaped colonies are closer to 0 and round colonies...

Страница 135: ...some shading for clearer visualization Select the Display Proximity Indicators check box to display connecting red lines between a detected colony and its closest neighbor Select the Shade Exclusion...

Страница 136: ...Finished Picking Current Batch dialog displays Continuing or Ending the Regional Picking Routine After all the colonies are picked from the source receptacles and delivered to the destination plates...

Страница 137: ...tination plates used and missing source receptacles To save this information in csv format click Export To view details of all activities related to the source and destination receptacles click Detail...

Страница 138: ...QPix 420 Colony Picking System User Guide 138 5031496 G...

Страница 139: ...nal process See Blue White Regional Picking Processes on page 119 Control Plate Creation creates a batch of plates containing control samples These can be created by picking colonies from source recep...

Страница 140: ...are included in these procedures might not be available depending on the features included with the instrument and license Opening the Control Plate Creation Window To open the Control Plate Creation...

Страница 141: ...ting Routines Before you can import a routine you must export the routine from the other computer in xml format and then save the export file on the system computer To import the xml file click Import...

Страница 142: ...r Devices to add a new plate type to the list See Obtaining Support on page 209 2 Select either to dip the pins a number of times or to stir the wells of the destination plate during inoculation To di...

Страница 143: ...ick Next to define options for the source receptacle Setting Source Receptacle Options To set source and receptacle options 1 In the Source window from the Holder list select the type of holder used t...

Страница 144: ...wells To deselect a well click the well again Wells that have not been selected show in light blue 3 Continue clicking receptacles and wells until all the control wells have been defined 4 Click Next...

Страница 145: ...K to move the actuator into position near the front of the instrument 3 Open the front door 4 Install the picking head To remove an installed head unscrew and remove the thumbscrew and washer on the l...

Страница 146: ...ecting Control Plate Creation Routines on page 140 If you do not need to change the routine select the Skip Steps check box before you click Next 3 Review the configured settings for the routine See V...

Страница 147: ...n within the image 1 Right click over the image map and select Intensity 2 Move the cursor over the area of interest in the image map to display the pixel intensity value To adjust the test image 1 Fr...

Страница 148: ...the value from the Threshold field into the Low Limit field To determine the High Limit value clear the Use Auto Thresholding check box and then drag the slider to the right until some colonies start...

Страница 149: ...ion window pickable objects show in yellow and unpickable object show in red A colony can be considered unpickable if it is too close to the edge of the receptacle or it does not match the selection c...

Страница 150: ...colonies to pick so that when picking one colony a different adjacent colony is not picked The system default value is 0 45 mm 3 Manually change the pickable property of individual objects To define...

Страница 151: ...e Control Plate Creation Progress While the control plate creation routine is running the Picking Progress window displays a summary of the routine Start Time shows the time that the picking of the co...

Страница 152: ...strument deck Note Make sure that the source receptacles are in the exact same tray locations so that the instrument can locate them 5 Close the instrument door 6 Click Next to continue running the pi...

Страница 153: ...sing the regional process Blue White Regional Picking Processes on page 119 Control Plate Creation creates a batch of plates that contain control samples These can be created by picking colonies from...

Страница 154: ...in the Regional Tray Name field 3 Complete all the parameter fields on the left side of the window using the specification document from the manufacturer Failure to input correct plate dimensions can...

Страница 155: ...Regional Trays icon The new plate definition screen displays along with parameter fields and a guide image 2 Select the Manage Previously Created Trays tab 3 From the Defined Trays list select the na...

Страница 156: ...QPix 420 Colony Picking System User Guide 156 5031496 G...

Страница 157: ...Editing Replication Processes The procedures for creating and editing the different types of replicating processes are similar Differences and exceptions are identified within the instructions when a...

Страница 158: ...n the Routines window select to create a new routine or to edit an existing routine To create a new routine click New Routine To edit an existing routine click Run Existing Routine and then select the...

Страница 159: ...s the one you are creating then the imported routine displays in the Select Routine list Exporting a Routine To export a routine click Export and then navigate to the location that you want to save th...

Страница 160: ...e for a barcode Note If the system fails to identify a barcode for the source receptacle the routine ends since the source requires a valid barcode Click Auto Generate to allow the system to automatic...

Страница 161: ...ntifiers for the source receptacles from a text or csv file click Import and then select the file from which to import the identifiers To remove an identifier from the list click the identifier in the...

Страница 162: ...ary Compression you can select 384 well plates only For Library Expansion you can select 96 well plates only 4 Select either to dip the pins a number of times or to stir the wells of the destination p...

Страница 163: ...Position to move the picking head arm to the loading position 2 When the message displays make sure that the bed is clear of obstructions and that the door is closed and then click OK to move the actu...

Страница 164: ...ing all of your changes click Next CAUTION At this point you cannot go back and change settings To make changes now you must cancel the process and start again 5 Remove all plate lids 6 In the Load Pl...

Страница 165: ...mmary window click Next 5 In the Continue or Save New Routine dialog or the Save Changes to Routine dialog select whether or not to save the routine before continuing with the replication process If y...

Страница 166: ...n plate into which the A1 pin is being lowered This value changes for 384 well plates Calculating Remaining Time indicates remaining time in the routine Current Action displays what the system is curr...

Страница 167: ...and Editing Sanitise Profiles on page 33 Creating and editing a rearraying process involves the following procedures Opening the Rearraying Window on page 167 Selecting Rearraying Routines on page 168...

Страница 168: ...tine select the routine in the Select Routine list and then click Delete In the Delete Routine dialog click OK to permanently remove the routine from the database Selecting Barcode Options You can sca...

Страница 169: ...right click the source plate and then click Add Selected Barcode If you add a plate to the Selected Barcodes list that is not a source for this routine then select the plate and click Remove When all...

Страница 170: ...not listed contact Molecular Devices to add a new plate type to the list See Obtaining Support on page 209 2 Select either to dip the pins a number of times or to stir the wells of the destination pl...

Страница 171: ...options are configured correctly for the rearraying routine If you must make changes click Back until you return to the window where the changes can be made To print the summary click Print Click Next...

Страница 172: ...screw to secure the head to the actuator 5 Close the front door 6 Click Move to Park Position to move the picking head arm to the run ready park position 7 When the message displays make sure that the...

Страница 173: ...Settings Summary window click Next 5 In the Continue or Save New Routine dialog or the Save Changes to Routine dialog select whether or not to save the routine before continuing with the rearraying pr...

Страница 174: ...r of wells that have been rearrayed Total Wells to Rearray shows the total number of wells to be rearrayed Estimated Time Remaining indicates remaining time in the routine Current Action displays what...

Страница 175: ...nitise Profiles on page 33 Creating and editing a gridding process involves the following procedures Opening the Gridding Window on page 175 Selecting Gridding Routines on page 176 Selecting Barcode O...

Страница 176: ...a Routine To delete a routine select the routine in the Select Routine list and then click Delete In the Delete Routine dialog click OK to remove the routine from the database Selecting Barcode Optio...

Страница 177: ...identifiers with the prefix Auto for the source receptacles select the Generate Random Barcodes check box No other parameters are required for this option To manually define identifiers for the source...

Страница 178: ...Dips field enter the number of times to dip the pins To stir the wells click Stir Source 3 In the Inoculation Height mm field enter the distance in millimeters mm above the bottom of the plate where t...

Страница 179: ...to a minimum of 96 samples on your destination surface The maximum number of samples can be defined with 25 spots in the Spot Pattern and 6 fields in the Field Pattern that requires 150 filled 384 wel...

Страница 180: ...of the spot size in the Estimated Spot Size m field enter a value in microns up to 1500 5 Under Structure field enter the number of Rows and Columns to stamp in the Spot Pattern for each pin 6 In the...

Страница 181: ...The field pattern must have a logical numerical sequence to it such as 1 2 3 4 5 6 or 1 2 2 3 3 4 If you create an illogical sequence such as 1 1 2 5 3 5 the No Sequence button displays in red For inf...

Страница 182: ...d four times and re inked after two stamps the Immediate method stamps the first spot two times before returning to the source plate to collect more sample and then stamps the second spot two times be...

Страница 183: ...e actuator Before storing the head or whenever it needs cleaning wipe the exterior of the head with a cloth using 70 ethanol To store the head slide it into its metal cover with the pins facing inward...

Страница 184: ...he routine 4 In the Settings Summary window click Next 5 In the Continue or Save New Routine dialog or the Save Changes to Routine dialog select whether or not to save the routine before you continue...

Страница 185: ...e current destination spot replicate onto which the sample is being stamped Stamp shows the current stamp being gridded Estimated Time Remaining shows an estimate of the time remaining in the gridding...

Страница 186: ...QPix 420 Colony Picking System User Guide 186 5031496 G...

Страница 187: ...s to Routines Receptacles and Locations on page 190 Exporting Data and Settings on page 191 Working With Plate Properties on page 191 Viewing Receptacle Location Maps on page 192 Working With Sample T...

Страница 188: ...d then from the Available Tags list select the tag you want and click Add to add the tag to the Search Tags list You can add as many tags as you need The items related to the selected tags display See...

Страница 189: ...tions 1 Find and select the routine receptacle or location to which you want to add tags See Finding Data in the Database on page 187 2 From the list on the left click Add Tag 3 In the Select Tags to...

Страница 190: ...hich you want to add an annotation See Finding Data in the Database on page 187 2 From the list on the left click Add Annotation 3 In the Add Annotations dialog enter the annotation 4 Click either Pro...

Страница 191: ...the list on the left click Add Property 3 In the Add Property dialog in the Property Name field enter a name for the property 4 In the Property Value field enter the value for the property 5 From the...

Страница 192: ...king With Sample Trails You can use a sample trail to display the details of how one or more wells were used in a plate for a routine Add wells to the Sample Trail list in the lower right corner of th...

Страница 193: ...2 From the Sample Trail list in the lower right area of the Data Viewer window click Add Remove 3 In the Edit Sample Trail dialog click the wells to include in the sample trail Unselected wells are y...

Страница 194: ...QPix 420 Colony Picking System User Guide 194 5031496 G...

Страница 195: ...tenance procedures other than those specified in this guide must be performed by Molecular Devices When service is required contact Molecular Devices technical support Doing Preventive Maintenance You...

Страница 196: ...lution Spor Klenz Ready To Use Cold Sterilant US EU Asia or ParaSafe EU The instrument can be left in a laboratory during formaldehyde vapor fumigation at a safe concentration However excessive formal...

Страница 197: ...ecommends that you spray ethanol on a cloth for cleaning the instrument Autoclaving is not compatible with anodized parts Do not use the ethanol on acrylic parts Use water and then turn on the UV lamp...

Страница 198: ...s metal cover with the pins facing inward and then secure it in place with the thumbscrew and washer 3 Remove the 10 screws that secure the top plate to the head CAUTION Use care to not damage the scr...

Страница 199: ...own in the instrument with the UV lamp on CAUTION Do not autoclave the anodized metal picking head You can soak the springs and screws in 100 ethanol and then let them thoroughly dry Replacing the Pic...

Страница 200: ...n Fire Test checks that the pins on an installed head are obstruction free and can move freely To test the pins 1 From the Navigation window under Utility Processes double click the Instrument Utiliti...

Страница 201: ...nues until you click Stop or all the pins have been fired The image in the window indicates which pin is fired by clearing the dot from the circle for its position on the picking head To define the sp...

Страница 202: ...ocesses double click the Camera Alignment Process icon and wait for the Camera Alignment Process window to display 2 If you do not have a standard 96 pin picking head installed click Move to Load Posi...

Страница 203: ...image is clearly visible 11 Click Fire Pin A1 and then click the blue arrows on the left to move the pin down until it creates a visible indent in the agar You can adjust the increment for each movem...

Страница 204: ...er replace the fuses You can obtain replacement fuses from Molecular Devices Fuses must be replaced with the correct type and rating as specified in Technical Specifications on page 219 CAUTION Do not...

Страница 205: ...ce a new fuse into the carrier by hand 6 Slide the fuse carrier back into the instrument 7 Use the screwdriver to turn the carrier clockwise until it is snug but do not over tighten it 8 Plug the powe...

Страница 206: ...n and tube attached 2 Unscrew the fluid collection bottle cap and dispose of the fluid as required by your lab protocol 3 Screw the fluid collection bottle cap back on and return the bottle to the met...

Страница 207: ...eptacleVault Security Users 13 Confirm that the new user name is included in the list 14 Close the Microsoft SQL Server Management Studio window Running the Software as an Administrator The QPix 420 C...

Страница 208: ...ed ConnectionString 6 Set the Connection String value so that the new computer name is included in the path statement Data Source COMPUTERNAME sqlexpress Initial Catalog ReceptacleVault Integrated Sec...

Страница 209: ...cal notes software upgrades safety data sheets and other resources If you still need assistance you can submit a request to Molecular Devices Technical Support Please have your instrument serial numbe...

Страница 210: ...QPix 420 Colony Picking System User Guide 210 5031496 G...

Страница 211: ...puter does not start Make sure that the power switch on the computer is in the on position and that the main power switch is turned on at the wall Make sure that the fuses are in good condition See Re...

Страница 212: ...he edge of the bioassay tray Check the agar volume setting bioassay tray positioning and tray holder positioning in the bed A Door Open warning displays Make sure that the door is closed and locked Lo...

Страница 213: ...ular Devices Technical Support if you need to change the scan direction on your instrument If one out of 10 plates fails to be read check the placement of the barcode label on the plate This might be...

Страница 214: ...QPix 420 Colony Picking System User Guide 214 5031496 G...

Страница 215: ...tion Software Kit Includes software license and 2 filters X1101 S ME004541 Wash bath S ME004542 Wash brushes Note Replacements included with service contracts X4390 Picking springs 100 springs per pac...

Страница 216: ...4310A Re arraying and replicating head for E coli standard transfer 96 pin for 96 well and 384 well plates tip diameter 0 55 mm pin length 49 5 mm X4310B Re arraying and replicating head for Yeast sta...

Страница 217: ...well Petri Dishes Microplates and Holders X4451 Petri dish holder 4 hole X4452 Petri dish holder 1 hole X4453 Petri dish holder 5 hole X4454 OmniTray holder 2 hole X6023 Vented QTray with cover 242 m...

Страница 218: ...QPix 420 Colony Picking System User Guide 218 5031496 G...

Страница 219: ...nt maintain a 66 cm 26 in minimum clearance area on the right side of the instrument Ambient operating temperature 15 C to 40 C 59 F to 104 F Ambient storage and transport temperature 25 C to 55 C 13...

Страница 220: ...ume 80 L min Optional air compressor Compressor unit DynAir DA7001CS Clean oil free compressor with sub micron filtration Dimensions 440 mm width x 440 mm depth x 613 mm height Weight 38 kg 84 lb Powe...

Страница 221: ...nd inches 1 2 Item Description 1 Height of instrument 75 cm 30 in 2 Width of instrument 152 cm 60 in Front View of the QPix 420 Colony Picking System with Dimensions 2 1 Item Description 1 Height of i...

Страница 222: ...QPix 420 Colony Picking System User Guide 222 5031496 G...

Страница 223: ...CC Notice This equipment has been tested and found to comply with the limits for a Class A digital device pursuant to part 18 of the FCC Rules These limits are designed to provide reasonable protectio...

Страница 224: ...QPix 420 Colony Picking System User Guide 224 5031496 G...

Страница 225: ...ecular Devices LLC or their respective owners Specifications subject to change without notice Patents www moleculardevices com patents FOR RESEARCH USE ONLY NOT FOR USE IN DIAGNOSTIC PROCEDURES 2022 M...

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