Molecular Devices QPix 420, Руководство пользователя

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Chapter 11: Control Plate Creation Processes
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Adjusting the White Light Test Image
In the Test Image window, all detected features are viewed as potential colonies to pick for
inoculation of the destination plates. To make sure that only colonies are detected, adjust the
test image in this window using the Exposure and Gain settings, the Threshold settings, and the
Debris settings. Each detected feature displays within a yellow ring.
To view the pixel intensity values in the image map to manually set a detection threshold or
detect areas of saturation within the image:
1. Right-click over the image map and select Intensity.
2. Move the cursor over the area of interest in the image map to display the pixel intensity
value.
To adjust the test image:
1. From the Receptacle list, select the number of the receptacle to view and then click the
frame to view in the receptacle image below the list. The frame is in red.
2. Under Acquisition, adjust the Exposure and Gain settings.
In the Exposure (ms) field, enter the number of milliseconds (from 10 to 1000) to expose
the sample to light when acquiring the image.
Adjust the Gain to increase the image signal without changing the light exposure.
After you adjust the Exposure or Gain settings, click Grab Image to apply the new settings
to the test image.
3. Select the Detection tab.