Molecular Devices QPix 420, Руководство пользователя

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Chapter 7: Blue-White Picking Processes
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9. Adjust the Diameter and Axis Ratio to exclude objects that are smaller than the desired
colonies. Each detected feature displays within a yellow ring while excluded features do not
have a yellow ring.
In the Diameter (mm) field enter a value in millimeters for the minimum diameter of the
required colonies.
In the Axis Ratio field, define the minimum roundness ratio of the required colonies.
The Features Found field displays the number of objects detected as colonies. The value
changes as you make adjustments.
Note: Further refinement for colony detection can be done on a higher-resolution
image in the Feature Selection window.
10. Click Next to go to the next step.
Selecting Blue-White Colonies for Picking
After you adjust and refine test images, the system captures and processes a higher-resolution
image using the test-image adjustments and then displays the Feature Selection window.
In the Feature Selection window, pickable objects show in yellow and unpickable object show
in red outline. A colony can be considered unpickable if it is too close to the edge of the
receptacle or it does not match the selection criteria.
To view the details of a colony, hold the cursor over a colony to display the properties of that
colony. If a colony has not been selected as pickable, the reason for the exclusion shows in red
text.